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Carcinoembryonic Antigen (CEA) antibody

Antigen

Carcinoembryonic Antigen (CEA)
Synonyms CEACAM5, CEA, CEACAM, CD66e, DKFZp781M2392, Psg30, 1600029H12Rik
Clonality Monoclonal (1B2)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (IHC)
Catalog no. ABIN180034
Quantity 500 µg  (Variants)
Price 517.00 $   Plus shipping costs $35.00
Shipping to
Availability Ships within 10 to 15 Business Days

Additional Information
Application Details Alternatives Variants back to top

Alternative name CEA (Carcinoembryonic Antigen)
Immunogen Human CEA
Isotype IgG2a
Clone 1B2
Description Carcinoembryonic antigen (CEA) was reported as cancer-specific antigen existing in human colon cancer tissue and embryo intestinal tract in 1965. However it was denied that the antigen is carcinoembryonic later. CEA exists in the blood of patients with some benign disease as well as with cancer disease. The production of CEA is observed in many cancer tissues and the CEA value in the blood reflects tumor size. Therefore, the assay is widely used in a diagnosis and in monitoring of cancer. CEA is glycoprotein which has molecular weight of about 180kDa, and is detected as broad band in globlin area in electrophoresis. The bands are due to diversity of the carbohydrate portion and the carbohydrate content in CEA is 50~60% The CEA includes CEA related antigens which do not crossreact with CEA antisera such as NCA (non-specific cross reacting antigen) derived from lung and spleen, NCA-2 derived from the embryo stool, NFA-1 (normal fecal antigen-1) derived from the normal adult stool and NFA-2. This product is CEA-specific antibody which does not crossreact with NCA.
Specificity Does not crossreact with with NCA

Application Details
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Application Notes This antibody can be stained in formalin fixed paraffin embedded tissues by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) method. The optimal dilution is about 4~10 ug/ml, however, the dilution rate should be optimized by each laboratories.
Purification Protein A
Buffer Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml
Storage Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20
Research Area Cancer
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "Carcinoembryonic Antigen (CEA)", type "Antibodies"
Hosts Mouse (65), Rabbit (38), Goat (6)
Reactivities Human (84), Mouse (Murine) (15), Rat (Rattus) (15), Cow (Bovine) (10), Dog (Canine) (9), Pig (Porcine) (5), Horse (Equine) (3), Chicken (2), Rabbit (2), Cat (Feline) (1), Goat (1), Lizard (1), Zebrafish (Brachydanio rerio) (1)
Applications Flow Cytometry (FACS) (32), Immunohistochemistry (IHC) (30), Immunofluorescence (IF) (28), ELISA (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Western Blotting (WB) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (7), Immunoprecipitation (IP) (7), Radioimmunoassay (RIA) (4), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), To be determined by user (TBD) (1)
Conjugates HRP (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (3), PE,Cy5.5 (3), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
Epitopes Center (1)