Presenilin 1 (PSEN1) antibody

Antigen: Presenilin 1 (PSEN1)

Synonyms: AD3, FAD, PS1, S182, Ad3h, PS-1, pre1, psen, zfPS1, zf-PS1, PSEN1, ad3, fad, ps1, s182, X-PS-beta, X-PS-alpha, DKFZp469K2026

Clonality: Monoclonal (17C2)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180044

Additional Information

Alternative name: Presenilin-1

Immunogen: N-terminal fragment of recombinant Human Presenilin-1 (E. coli)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 17C2

Description: The presenilin 1 (PS1) gene was identified as the gene that harbors mutations that cause this type of FAD (termed AD3). PS1 is a novel 467-aa long integral membrane protein localized to intracellular membranous compartments, especially the endoplasmic reticulum, that spans the membrane 7-9 times. More than 30 missense mutations or amino acid deletions have been identified in a number of AD3 pedigrees. Shortly after the discovery of PS1, the gene responsible for FAD was found on chromosome 1. This gene is known as PS2 and it is highly homologous to PS1 (67% identical). Sequence analysis predicts integral membrane proteins, which contain seven putative transmembrane domains, a short hydrophilic amino- and carboxyl-terminal tail, and a large hydrophilic loop between the sixth and seventh membrane-spanning domain. All of reported mutation that results in early-onset Alzheimer's disease are missense mutations, 24 in PS1 [5-11] and two in PS2 [12, 13], or mutations that affect the splicing without affecting the coding of the protein [14] and this has led the hypothesis that they may result in a gain of (miss) function.

Specificity: Human Presenilin-1 specific, No crossreactivities with Human Presenilin-2

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment following formic acid treatment*1 by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 1-0 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of 5 ug /ml. *1 Rinsing by running water after formic acid treatment for 5 minutes following de-paraffin.

Purification: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1 ml distilled water will be added to the product

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Research Area: Alzheimer's Disease

Restrictions: For Research Use only

Images

anti-Presenilin 1 (PSEN1) antibody

Human brain (Alzheimer's disease)