Osteopontin (SPP1) antibody

Antigen: Osteopontin (SPP1)

Synonyms: OPN, BNSP, BSPI, ETA-1, MGC110940

Clonality: Monoclonal (1B20)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180047

Additional Information

Alternative name: Osteopontin

Immunogen: Synthetic peptide in portion of C-terminus of Human Osteopontin

Isotype: IgG  (Matching secondary antibodies)

Clone: 1B20

Description: Osteopontin (OPN) is a secreted glycoprotein that was originally isolated from bone. Its molecular weights have been reported in the range of 66 kDa to 44 kDa depending on glycosylation and phosphorylation. OPN is also known to be expressed in other fluids and tissues including milk, urine, activated T cells, smooth muscle cells, kidney and some tumor cells. OPN contains an Arg-Gly-Asp (RGD) amino acid sequence. This motif is present in fibronectin, vitronectin and a variety of other extra-cellular proteins that bind members of the integrin family of cell surface receptors such as a v3. Recently OPN was identified as a ligand for CD44, which levels correlate with aggressiveness of lymphoid tumors and invasiveness of bladder carcinoma. Its interaction does not require RGD motif of OPN. In OPN knockout mice, it has been reported that a significantly decreased level of debridement was shown. Although the distribution and expression pattern of OPN in the human body have suggested the multiple function of OPN, its function under different situations remain obscure.

Specificity: The antibody, interestingly, can be stained with rat osteopontin. Can react with human osteopontin, but unknown staining is observed in erythrocytes. (This reaction cannot be seen in rat.) crossreactivities with rabbit plasma osteopontin.

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 1-2 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of about 2 ug/ml.

Purification: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 Reconstitution: 1 ml distilled water will be added to the product

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only

Images

anti-Osteopontin (SPP1) antibody