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NapsinA (TMU-Ad02) antibody
| Antigen | NapsinA (TMU-Ad02) |
| Clonality | Monoclonal (TMU-Ad02) |
| Host |
Mouse |
| Reactivity |
Human |
| Application |
Immunohistochemistry (IHC), Western Blotting (WB)
|
| Catalog no. | ABIN180050 |
| Quantity | 50 µg |
| Price | 517.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 10 to 15 Business Days |
Additional Information
| Immunogen | Synthetic peptide for a part of N terminus of Human Napsin A |
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | TMU-Ad02 |
| Description | Recently, a new protein isolated by two-dimensional immuno-electrophoresis from tissue of lung adenocarcinoma was identified as Napsin A, a new aspartic Proteinase. The reactivity of the antibody showed immunohistochemically in type II pneumocytes, alveolar macrophages, renal tubules and exocrine glands and ducts in pancreas. In particular, type II pneumocytes and alveolar macrophages showed strong staining. The specificity of the antibody is very interesting, which in primary lung adenocarcinoma, 47 out of 58 (81.0%) primary lesions were positive. All well-differentiated adenocarcinoma except those of goblet type showed strong staining. The antibody may be a useful tool as a tumor marker for primary lung adenocarcinoma. |
| Specificity | Immunohistochemical study in histopathological types of lung carcinoma Expression levels of the antibody Histology 2+ + +/- - Positive Rate (%) Adenocarcinoma 25 11 11 11 81.0 Well-differentiated 16 3 0 0 100.0 Moderately differentiated 8 2 4 4 77.8 Poorly differentiated 1 6 7 7 66.7 Squamous cell carcinoma 0 0 0 14 0 Large cell carcinoma 0 0 2 5 28.6 Small cell carcinoma 0 0 0 9 0 Undifferentiated carcinoma 0 0 0 1 0 Carcinoid tumor 0 0 0 2 0 |
Application Details
| Application Notes | This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 0.51 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of 0.51 ug/ml. |
| Purification | Antigen peptide |
| Buffer | Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1 ml distilled water will be added to the product |
| Storage | Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20 |
| Research Area | Cancer |
| Restrictions | For Research Use only |
