Tau/Amino Junction (9A1) antibody

Antigen: Tau/Amino Junction (9A1)

Clonality: Monoclonal (9A1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180051

Additional Information

Immunogen: Synthetic peptide of part of Human Tau/Amino Junction (DTDAGLKAEEAGIG)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9A1

Description: Tau protein is a microtubule-associated protein expressed in neuroaxon and has molecular weight of 50~70 kDa. Some Alzheimer's patients have sites where tau protein is accumulated abnormally. It is known that six isoforms of tau protein formed by alternative splicing of tau gene located on chromosome 17. This monoclonal antibody can specifically recognize junction between exon 1 and 4 of both Tau 352 and Tau 383, and it is useful for the study of brain research.

Specificity: This antibody react with Tau isoforms as following figure: Side of C terminal Tau 3 repeat Tau 4 repeat Side of N terminal Tau 352 Tau 381Tau 410Tau 383Tau 412 Tau 441

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues after formic acid treatment by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 1 ug/ml, however, the dilution rate should be optimized by each laboratories. Rinsing by running water after formic acid treatment for 5 minutes following de-paraffin. This antibody can be used for western blotting in concentration of 1 µg/mL

Concentration: 1.0 mL deionized water will be added to the product, then its concentration comes to 100 μg/mL

Purification: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 Reconstitution: 1.0 ml distilled water will be added to the product, then the concentration comes to 100 ug/ml

Storage: Lyophilized product, 5 years at 2-8°C, Solution: 2 years at ­20°C

Research Area: Alzheimer's Disease

Restrictions: For Research Use only

Images

Human Brain (Alzheimer's Disease)

anti-Tau/Amino Junction (9A1) antibody (Image 2)