Tau/Repeat Domain (2B11) antibody

Antigen: Tau/Repeat Domain (2B11)

Clonality: Monoclonal (2B11)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180053

Additional Information

Immunogen: Synthetic peptide of the Repeat Domain of human Tau (PGGGKVQIVYKP)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2B11

Description: Tau protein is a microtubule-associated protein expressed in neuroaxon and has molecular weight of 50~70 kDa. Some Alzheimer's patients have sites where tau protein is accumulated abnormally. It is known that six isoforms of tau protein formed by alternative splicing of tau gene located on chromosome 17. This monoclonal antibody can specifically recognize junction between exon 1 and 4 of both Tau 352 and Tau 383, and it is useful for the study of brain research.

Comments:

Source : Mouse-Mouse hybridoma (X63 - Ag 8.653 × BALB/c mouse spleen cells, supernatant)

How to use : 1.0 mL deionized water will be added to the product, then its concentration comes to 100 μg/mL.

Application Details

Application Notes: This antibody can be used for immunohistochemistry with formalin fixed paraffin embedded tissues after formic acid treatment (rinsing by running water after formic acid treatment for 5 minutes following de-paraffin), by several techniques such as Avidin Biotin Complex (ABC) Method. The optimal concentration is 5 μg/mL, however, the concentration should be optimized by each laboratory. This antibody can be used for western blotting in concentration of 3 - 5 μg/mL.

Purification: Affinity purified with anti-mouse IgG

Buffer: Lyophilized product from 1 % BSA in PBS containing 0.05 % NaN3

Storage: Lyophilized product, 5 years at 2-8 °C. Solution, 2 years at –20 °C.

Research Area: Alzheimer's Disease

Restrictions: For Research Use only

Images

Human Brain (Alzheimer's Disease)

anti-Tau/Repeat Domain (2B11) antibody (Image 2)