Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) antibody

Antigen: Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2)

Synonyms: PDJ, PRKN, AR-JP, LPRS2, SD01679, dpk, Dmel\CG10523, CG10523, Park, Prkn, zgc:112390, PARK2, pdr-1, LOC100150461

Clonality: Monoclonal (5A1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN180055

Additional Information

Alternative name: Parkin (5A1)

Immunogen: Synthetic peptide of part of C terminal of human parkin (AYRVDERAAEQARWEAA)

Isotype: IgG1

Clone: 5A1

Description: Parkinson's Disease (PD) is a relatively common neurodegenerative disorder, which is characterized by the loss of midbrain dopamine (DA) neurons and the presence of Lewy bodies, proteinaceous cytoplasmic inclusions that are abundantly enriched in ubiquitin. It is identified a number of potential substrates for parkin, which may be involved in the pathogenesis of PD. Autosomal Recessive Juvenile Parkinsonism (AR-JP) is a recently described form of Parkinson's Disease that has been linked to a gene that codes for parkin. Parkin, a 52 kDa protein, has a suggested role in the ubiquitin/proteasome pathway for protein degradation. The amino terminus bears sequence homology to ubiquitin while functionally it acts as a RING-type ubiquitin protein ligase (E3) that coordinates the transfer of ubiquitin to substrate proteins, thus targeting them for degradation by the proteasome.

Specificity: Specifically reacts with parkin cDNA transfected COS cells by immuno- cytochemistry. Does not crossreact with with rat, but not tested with mouse.

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment (10min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 5 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of about 5 ug/ml.

Purification: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only

Images

anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) antibody

anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) antibody (Image 2)