Soluble beta Amyloid Precursor Protein (Swedish) (sAPPb) antibody

Antigen: Soluble beta Amyloid Precursor Protein (Swedish) (sAPPb)

Clonality: Monoclonal (6A1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180059

Additional Information

Alternative name: sAPPb (soluble beta Amyloid Precursor Protein -Swedish )

Immunogen: Synthetic peptide for C-Terminal of Human sAPP-sw (ISEVNL)

Isotype: IgG3  (Matching secondary antibodies)

Clone: 6A1

Description: Amyloid is derived by the sequential cleavage of amyloid precursor protein (APP) by beta- and gamma-secretases. A double missense mutation (Lys670Asn and Met671Leu) in APP found in a Swedish pedigree (APP-sw) elevates Abeta40 and Abeta42 production (1), and the mutation is utilized in establishment of transgenic mice overexpress a mutant form of human amyloid precursor protein (2). Amyloidproduction and, beta-secretase cleavage of APP -sw reportedly occur in the endoplasmic reticulum (ER), Golgi and endocytic compartments (3).

Specificity: The antibody is specific for soluble Amyloid Precursor Protein of Swedish type cleaved by beta-secretase. No cross reactivities with sAPPbeta-sw of wild type and the full length APP of both Swedish and wild types.

Application Details

Application Notes: This antibody can be stained in sAPP-sw gene transfected COS cells after fixation by 20% formalin containing methanol solution without any pretreatment by several cytochemical techniques such as Avidin Biotin Complex (ABC) Method. On the other hand, the antibody can not stained sAPP-wild type gene transfected COS cells by same procedure. The optimal dilution is about 5 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of about 5 ug /ml.

Purification: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then the concentration comes to 100 ug/ml

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only

Images

anti-Soluble beta Amyloid Precursor Protein (Swedish) (sAPPb) antibody

Publications

Publications: Fu, Li, Luo et al.: "Promising anti-Alzheimer's dimer bis(7)-tacrine reduces beta-amyloid generation by directly inhibiting BACE-1 activity." in: Biochemical and biophysical research communications, Vol. 366, Issue 3, pp. 631-6, 2008 (PubMed).