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Antibodies
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anti-Tenascin C (TNC) (Domain B) antibody
Tenascin C (TNC) (Domain B) antibody
| Antigen |
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| Synonyms |
TN, HXB, MGC167029, Hxb, Ten, TN-C, AI528729, MGC144208, MGC144209, cytotactin, tenascin-C, C130033P17Rik, tenc, wu:fk04d02, TNC, tn, MGC140517 |
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Epitope
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| Clonality |
Monoclonal (4C8MS) |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
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| Catalog no. |
ABIN180062 |
| Quantity |
100 µg |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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Tenascin-C (Domain B)
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Immunogen
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Human Recombinant Tenascin-C
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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4C8MS
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Description
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Tenascin-C (TN-C) is a component of the extracellular matrix (ECM) that has been shown to be involved in tissue interactions during fetal development and oncogenesis. It is glycoprotein consisting of six disulphide monomer isoforms being generated by alternative splicing. A high molecular weight variant that is generated by alternative splicing of RNAs of TN-C was found predominantly in breast, prostatic and colorectal cancers. The appearance of such a large TN-C isoform has been suggested to be of significance for tumour progression. This monoclonal antibody can specifically be recognized domain B on FNIII repeats of TN-C, and it is useful for the study of organogenesis, tumor, cell injury caused by various types of stress, wound healing, regeneration and fibrosis.
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Specificity
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Specifically reacts with Domain B on FNIII repeats of TN-C Cross: Cross-reacts with Mouse and Rat Reactivity
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Application Notes
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The antibody can be used for immunohistochemical staining in concentration of about 5 ug/ml. The antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment (10mM Citrate Buffer, pH 6.0) by high-sensitive immunohistochemical techniques such as VECTASTAIN ABC Elite system (VECTOR), EnVision system (DAKO) and Max-Pro system (Nichirei). However, the antibody may not consistently stained by conventional system such as Avidin Biotin Complex (ABC) Method and LSAB Method. The dilution rate is only our recommendation, therefore, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of about 5 ug /ml.
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Purification
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Protein A
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then the concentration comes to 100 ug/ml
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Storage
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Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20
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Restrictions
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For Research Use only
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Alternatives for antigen "Tenascin C (TNC)", type "Antibodies"
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Hosts
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Mouse (18)
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Reactivities
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Human (15), Guinea Pig (1)
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Applications
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Western Blotting (WB) (15), Immunohistochemistry (Frozen Sections) (IHC (fro)) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunoprecipitation (IP) (6), Enzyme Immunoassay (EIA) (5), Immunohistochemistry (IHC) (3), ELISA (2), Immunofluorescence (IF) (2), Immunocytochemistry (ICC) (1), Radioimmunoassay (RIA) (1)
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Epitopes
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EGF Like Domain (1)
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