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Antibodies
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anti-Platelet-Derived Growth Factor Receptor, beta Polypeptide (PDGFRB) antibody
Platelet-Derived Growth Factor Receptor, beta Polypeptide (PDGFRB) antibody
| Antigen |
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| Clonality |
Monoclonal (22B1) |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Neutralization (Neut)
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| Catalog no. |
ABIN180068 |
| Quantity |
100 µg |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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PDGFR
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Immunogen
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Recombinant protein of extracellular domain of human Tie-1
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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22B1
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Description
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TIE is a new receptor-type tyrosine kinase that was cloned from K562, a chronic myelocytic leukemia cell line. Its molecular weight is 117 kDa and its structure begins with an immunoglobulin-like domain from the extracellular N terminal, continues to 3 EGF-like domains, another immunoglobulin-like domain, 3 fibronectin III-like domains, a membrane-penetrating domain, 2 tyrosine kinase domains and a C-terminal domain. It has been reported that Tek molecules with a high homology (about 80%) have been cloned from the kinase domains. These molecules are believed to make up a Tie family. Tie-2 and a receptor type tyrosine kinase Tie-1 that is expressed specifically in endothelial cells are included in this Tie family. Among the Tie-1 gene knockout mice, a vasculature is formed in a homozygote (-/-) but the mouse is likely to succumb to pulmonary edema. Thus it has been reported that the signal via Tie-1 is important in maintaining the vascular structure. This monoclonal antibody recognizes Tie-1 by W.B. and I.P. applications and it enables FACS analysis of Tie-1-expressing cells.
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Specificity
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Confirmed by western blotting with HEL (leukemia) cell.
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Application Notes
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This antibody can be used for western blotting at 1-5 ug/mL. This antibody can be used for immunoprecipitation at 3-5 ug/mL.
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Purification
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Protein A
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 mL deionized water will be added to the product
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Storage
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Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20
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Restrictions
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For Research Use only
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Alternatives for antigen "Platelet-Derived Growth Factor Receptor, beta Polypeptide (PDGFRB)", type "Antibodies"
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Hosts
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Rabbit (4), Mouse (2)
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Reactivities
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Human (5), Mouse (Murine) (2), Rat (Rattus) (1)
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Applications
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Western Blotting (WB) (4), Enzyme Immunoassay (EIA) (2), ELISA (1), Flow Cytometry (FACS) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunoprecipitation (IP) (1)
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Epitopes
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C-Term (1), N-Term (1), pTyr740 (1), pTyr751 (1)
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