S-100 Protein (alpha chain) antibody

Antigen: S-100 Protein (alpha chain)

Clonality: Monoclonal (2A10)

Host: Mouse

Reactivity: Cow (Bovine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180077

Additional Information

Alternative name: S-100 Protein

Immunogen: Bovine Brain S-100 Protein

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2A10

Description: S100 proteins (16 members) show a very divergent pattern of cell- and tissue-specific expression, of subcellular localizations and relocations, of post-translational modifications, and of affinities for Ca2+, Zn2+, and Cu2+, consistent with their pleiotropic intra- and extracellular functions. Up to 40 target proteins are reported to interact with S100 proteins and for S100A1 alone 15 target proteins are presently known. Therefore it is not surprising that many functional roles have been proposed and that several human disorders such as cancer, neurodegenerative diseases, cardiomyopathies, inflammations, diabetes, and allergies are associated with an altered expression of S100 proteins.

Specificity: React to chain of Bovine and Human S-100 protein

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues after microwave pretreatment (10 min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Streptavidin-Biotin (SAB) method. The optimal dilution is about 2~5 ug/ml, however, the dilution rate should be optimized by each laboratories.:This antibody can be used for western blotting in concentration of about 2~5 ug/ml.

Purification: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product.

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only