kappa Light Chain antibody
| Antigen |
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| Synonyms |
LOC100037688 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
Flow Cytometry (FACS) (29), Immunofluorescence (IF) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (7), Western Blotting (WB) (6), Enzyme Immunoassay (EIA) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)
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| Catalog no. |
ABIN180112 |
| Quantity |
100 µg |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Immunogen
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Purified Human Kappa light chain
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Anti-Human IgG (goat IgG) is often used as a secondary antibody for the detection of Human IgG. Following recent advances in the development of antibody-based therapy, such as the development of Human antibodies, this secondary antibody has been used for the purpose of investigating metabolism in the living body following administration of this antibody. In studies using monkeys, conventional anti-Human IgG (goat IgG) antibody tends to cross-react with the monkeys' own antibody, making it difficult to achieve highly specific detection of the administered antibody. To overcome this problem, we have incorporated the additional step of absorption with monkey IgG. This is expected to minimize non-specific reaction even when the antibody is used for ELISA on monkey blood samples, and also make it possible to detect the antibody in a concentration range that is difficult to detect with conventional secondary antibodies.
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Specificity
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Human IgG 100% Monkey IgG less than 0.1%
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Application Notes
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This antibody can be used for ELISA in concentration of 1-5 ug/mL : This antibody can be used for immunohistochemistry in concentration of 1-5 ug/mL
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Purification
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Protein A and absorbed with Monky IgG
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 mL distilled water will be added to the product
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Storage
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Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20
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Restrictions
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For Research Use only
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Alternatives for antigen "kappa Light Chain", type "Antibodies"
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Hosts
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Rabbit (28), Mouse (17), Goat (5), Sheep (3), Rat (2)
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Reactivities
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Human (53), Mouse (Murine) (5), Cow (Bovine) (4), Dog (Canine) (2), Monkey (2), Pig (Porcine) (2), Rat (Rattus) (2)
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Applications
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Flow Cytometry (FACS) (29), Immunofluorescence (IF) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (15), ELISA (7), Western Blotting (WB) (6), Enzyme Immunoassay (EIA) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)
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Conjugates
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FITC (5), Biotin (3), PE (2), APC (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1), Texas Red (TR) (1)
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