Endothelin Big (0) (AA 22-38) antibody

Antigen: Endothelin Big (0)

Epitope: AA 22-38

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN180133

Additional Information

Alternative name: Endothelin Rat Big (22-38)

Immunogen: Synthetic peptides of rat big endothelin-1 (22-38) Purification: Affinity Purified with antigen peptides

Isotype: IgG  (Matching secondary antibodies)

Description: Endothelins are 21 amino acid peptides that are produced ubiquitously by vascular endothelial cells, smooth muscle cells, and other cells in different organs. Endothelins are secreted as big-endothelins that are converted to active proteins by the endothelin-converting enzyme. These peptides possess many biological activities, such as vasoconstriction and mitogenesis, and are involved in numerous physiological and pathophysiological processes in humans. Elevated plasma levels of endothelin have been associated with heart failure, and increased immunoreactivity for endothelin is observed in transplant coronary artery disease.

Specificity: Endothelin-1 0 % Endothelin-2 0 % Endothelin-3 0 % Big Endothelin-1 100.0 % Big Endothelin-2 <0.1 % Big Endothelin-3 <0.1 % VIC 0.1 % Rat Big Endothelin 100.0 %

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues by Immunohistochemical techniques such as SAB Method. The optimal dilution is 3·` 5 ug/ml, however, the dilution rate should be optimized by each laboratories.

Purification: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1 ml distilled water will be added to the product

Storage: Lyophilized product, 5 years at 2 ­ 8 C : Solution, 2 years at ­20 C

Restrictions: For Research Use only