TNF-a (Tumor Necrosis Factor) antibody

Antigen: TNF-a (Tumor Necrosis Factor)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180141

Additional Information

Immunogen: Synthetic peptide for C-Terminal of rat TNF Purification: Affinity Purified with synthetic peptide

Isotype: IgG  (Matching secondary antibodies)

Description: TNF (Tumor Necrosis Factor alpha) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-alpha has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-alpha is tightly controlled being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.

Application Details

Application Notes: This antibody can be stained in frozen sections by several Immuno- histochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 5 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of about 5 ug/ml.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product

Storage: Lyophilized product, 5 years at 2 ­ 8 C : Solution, 2 years at ­20 C

Restrictions: For Research Use only