Leptin (LEP) antibody
| Antigen |
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| Synonyms |
OB, OBS, FLJ94114, ob, obese |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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Western Blotting (WB) (95), Enzyme Immunoassay (EIA) (61), Immunofluorescence (IF) (61), Flow Cytometry (FACS) (55), ELISA (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Radioimmunoassay (RIA) (15), Immunoprecipitation (IP) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Transfected cell culture (TCC) (2), Blocking Antibody (Inhibition) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN180174 |
| Quantity |
100 µg (Variants) |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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Leptin
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Immunogen
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Synthetic peptide in portion of C-terminus of Mouse Leptin Purification: Purified with antigen peptide
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Isotype
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IgG (Matching secondary antibodies)
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Description
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The recessive obesity mutation, ob, identified in 1950, results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans. Freidman's group at Rockefeller University originally identified the obesity gene that encodes for Leptin in 1994. Leptin is a 16 KDa secreted protein produced by the ob gene. Adipose tissue produces Leptin and releases it into the bloodstream. As fat deposits grow, blood Leptin levels tends to increase. It has been suggested that Leptin acts as a lipostat, increasing as fat gets deposited into adipocytes. It is also found that the protein acts as a hormone instructing the brain to stop food consumption and to increase activity. The protein has also been shown to signal and probably control the onset of puberty. In addition, recent study shows Leptin acts as a potent inhibitor of bone formation through central nervous system.
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Specificity
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Cross-reacts with Rat
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Application Notes
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This antibody can be used for western blotting in concentration of 1~5 ug/ml.
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Purification
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Antigen peptide
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product
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Storage
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Lyophilized product, 5 years at 2 8 C : Solution, 2 years at 20 C
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Research Area
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Hormones
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Restrictions
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For Research Use only
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Alternatives for antigen "Leptin (LEP)", type "Antibodies"
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Hosts
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Rabbit (131), Mouse (36), Goat (6), Sheep (2)
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Reactivities
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Human (104), Mouse (Murine) (92), Rat (Rattus) (79), Pig (Porcine) (35), Cow (Bovine) (19), Dog (Canine) (18), Sheep (Ovine) (17), Horse (Equine) (15), Rabbit (2), Chicken (1)
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Applications
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Western Blotting (WB) (95), Enzyme Immunoassay (EIA) (61), Immunofluorescence (IF) (61), Flow Cytometry (FACS) (55), ELISA (30), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Radioimmunoassay (RIA) (15), Immunoprecipitation (IP) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Transfected cell culture (TCC) (2), Blocking Antibody (Inhibition) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
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Conjugates
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Biotin (15), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3)
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Epitopes
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C-Term (3), 92-145 cyclized (2), AA 1-24 (2), amino terminal residues 1-24 (2), mouse (1), rat (1), residues 131-145 (1)
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Alternatives 
