Prion Protein (PRNP) (N-Term) antibody

Antigen: Prion Protein (PRNP)

Synonyms: CJD, GSS, PrP, ASCR, PRIP, PrPc, CD230, prion, MGC26679, PrP27-30, PrP33-35C, Prn, MGC140197, prp, Prn-p, cjd, prp(c), MGC84114, PRNP, PRP, Prp, SIP, PRPC, DKFZp459A016

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180185

Additional Information

Alternative name: Prion Protein (N)

Immunogen: Synthetic peptide for N-terminal of Human Prion Protein Purification: Affinity Purified with synthetic peptide

Isotype: IgG  (Matching secondary antibodies)

Description: The prion protein (Prp) is a protein of unknown function that is expressed in both normal cells and in cells affected by Transmissible spongioform encephalopathies (TSEs) which are lethal neurodegenerative diseases affecting numerous mammals. TSEs are characterized by the conversion of the cellular protein PrPC to the disease-associated variant, PrPSC. The PrPC and PrPSC proteins share the same primary sequence and have no known posttranslational differences, and are believed to differ in folding conformation. PrpSC is found in high quantity in the brains of animals affected by TSEs, including scrapie in sheep, BSE in cattle and Creutzfeld-Jacob disease in humans.

Specificity: Confirmed by western blotting. Antigen peptide is 100% identical to Mouse, Rat, Gorilla and Chimpanzee. However, crossreactivities to those species are not tested.

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues after autoclave treatment*1 by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 0.5·` 1 ug/ml, however, the dilution rate should be optimized by each laboratories. *1 Specimen should be put in 1mM HCl during autoclave treatment (121·Z , 1-0min) This antibody can be used for western blotting at 0.5 ug/ml.

Purification: Antigen Peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 0.5 ml distilled water will be added to the product

Storage: Lyophilized product, 5 years at 2 ­ 8 C : Solution, 2 years at ­20 C

Restrictions: For Research Use only

Images

anti-Prion Protein (PRNP) (N-Term) antibody