Stratifin (SFN) antibody
| Antigen |
|
| Synonyms |
YWHAS, Er, Mme1, Ywhas, LOC408037, LOC100220844 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|
| Catalog no. |
ABIN180192 |
| Quantity |
100 µg (Variants) |
| Price |
517.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 10 to 15 Business Days |
|
Alternative name
|
14-3-3 sigma Protein (N)
|
|
Immunogen
|
Synthetic peptide of N-terminus portion for human 14-3-3 Sigma protein Purification: Affinity purified with synthetic peptide
|
|
Isotype
|
IgG (Matching secondary antibodies)
|
|
Description
|
The cell cycle checkpoint plays an important role in maintaining the integrity of cells. The G1 arrest is due to p53-mediated induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1/SDI1, but the basis for the G2 arrest is unknown. Recently, one of the 14-3-3 protein family members, 14-3-3sigma, was shown to be regulated by p53 and to play a role in the G2-M-phase checkpoint. 14-3-3 sigma was cloned by expression cloning through cyclin-dependent kinase 2 (CDK2) association. 14-3-3 sigma shares cyclin-CDK2 binding motifs with different cell cycle regulators, including p107, p130, p21 (CIP1), p27 (KIP1), and p57 (KIP2), and is associated with cyclin.
|
|
Specificity
|
Human 14-3-3 Sigma specific. No crossreactivities with 14-3-3 Beta, Gamma, Epsilon, Zeta, Eta, and Tau. (Confirmed by western blotting)
|
|
Application Notes
|
This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment (10 Min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 5 ug/ml, however, the dilution rate should be optimized by each laboratories.:This antibody can be used for western blotting in concentration of 1 - 3 ug/ml.
|
|
Purification
|
Antigen peptide
|
|
Buffer
|
Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product
|
|
Storage
|
Lyophilized product, 5 years at 2 8 C : Solution, 2 years at 20 C
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Stratifin (SFN)", type "Antibodies"
|
Hosts
|
Mouse (8), Rabbit (8), Goat (4)
|
|
Reactivities
|
Human (15), Mouse (Murine) (4), Rat (Rattus) (4), Dog (Canine) (2), Sheep (Ovine) (2), Chicken (1), Cow (Bovine) (1), Rabbit (1)
|
|
Applications
|
Western Blotting (WB) (19), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Immunohistochemistry (IHC) (7), Enzyme Immunoassay (EIA) (5), Immunoprecipitation (IP) (3), ELISA (1), ELISA (Detection) (1), Immunofluorescence (IF) (1)
|
|
Epitopes
|
Ab-1 (2), C-Term (1), N-Term (1)
|
