Single Stranded DNA (ssDNA) antibody

Antigen: Single Stranded DNA (ssDNA)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN180204

Additional Information

Immunogen: Bovine fragment single stranded DNA Purification: Purified with antigen

Isotype: IgG  (Matching secondary antibodies)

Description: Apoptosis, or programmed cell death, is a highly conserved biochemical mechanism that allows cells to die in a controlled and organized manner. This death process is essential for normal cellular differentiation and tissue homeostasis within multi-cellular organisms. The antibody can reacts with single stranded DNA, which produced on the process of nuclear fragmentation in apoptosis. In compare to TUNEL method, the immunohistochemical method using the antibody can be easily detects positive cells on apoptosis.

Specificity: Can reacts with random sequenced single DNA of all species not only of human

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues without any pre-treatment by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 0.5 - 1 ug/ml, however, the dilution rate should be optimized by each laboratories.

Purification: Antigen

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 200 ug/ml

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only

Images

Human tonsil

Publications

Publications: Oka, Ohno, Tsuchimoto et al.: "Two distinct pathways of cell death triggered by oxidative damage to nuclear and mitochondrial DNAs." in: The EMBO journal, Vol. 27, Issue 2, pp. 421-32, 2008 (PubMed).