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Syndecan 4 (SDC4) antibody
| Antigen |
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| Synonyms |
SYND4, MGC22217, Synd4, AA959608, AW108331, ryudocan, syndecan-4, RYUDOCA, RATRYUDOCA, SDC4, sdc4l, wu:fc47g09, amphiglycan, DKFZp469H1323 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Western Blotting (WB) (14), Immunoprecipitation (IP) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (4), ELISA (Detection) (4), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN180206 |
| Quantity |
100 µg |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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Syndecan-4
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Immunogen
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Maltose Binding Protein (MBP) - Syndecan-4 recombinant protein Purification: Purified with Recombinant Syndecan-4
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Syndecan, a cell surface proteoglycan, is an integral membrane protein acting as a receptor for the extracellular matrix. The syndecan family of four transmembrane heparan sulfate proteoglycans binds a variety of soluble and insoluble extracellular effectors. Syndecan extracellular domains (ectodomains) can be shed intact by proteolytic cleavage of their core proteins, yielding soluble proteoglycans that retain the binding properties of their cell surface precursors. The syndecan-4 acts in conjunction with the 51 integrin to promote the formation of actin stress fibers and focal adhesions in fibronectin-adherent cells.
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Specificity
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Confirmed by western blotting. Cross-Reactivities not tested
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Application Notes
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This antibody can be stained in formalin fixed paraffin embedded tissues after microwave pretreatment (10 min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) method. The optimal dilution is 2~4 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting at 1~2 ug/ml.
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Purification
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Antigen peptide
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml
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Storage
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Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20
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Restrictions
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For Research Use only
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Alternatives for antigen "Syndecan 4 (SDC4)", type "Antibodies"
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Hosts
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Rabbit (30), Mouse (2)
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Reactivities
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Human (31), Mouse (Murine) (25), Rat (Rattus) (25), Pig (Porcine) (19), Cow (Bovine) (18), Dog (Canine) (16), Chicken (3), Horse (Equine) (2), Monkey (1), Rabbit (1)
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Applications
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Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Western Blotting (WB) (14), Immunoprecipitation (IP) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), ELISA (4), ELISA (Detection) (4), Immunohistochemistry (IHC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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AA 1-50 (1)
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