Sirtuin 1 (SIRT1) antibody
| Antigen |
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| Synonyms |
SIR2L1, SIRT1, SIR2, sirtuin 1, SRT1 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)
Immunofluorescence (IF) (23), Western Blotting (WB) (23), Flow Cytometry (FACS) (20), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Enzyme Immunoassay (EIA) (6), Immunoprecipitation (IP) (4), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Chromatin Immunoprecipitation (ChiP) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN180207 |
| Quantity |
100 µg |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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Sir2/SIRT1 (Silent information Regulator 2)
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Immunogen
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Synthetic peptides for part of C Terminal of human Sir2/SIRT1 (LEDEPDVPERAGG) Purification: Purified with antigen peptides
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Imai et al. have found that S. cerevisiae Sir2p and its higher eukaryotic orthologs are novel nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylases (1). Sir2 proteins promote longevity in yeast and in C. elegans by silencing rDNA and regulating the insulin/IGF-1 signaling pathway, respectively. The enzymatic activities and functions of Sir2 proteins are highly conserved in evolution. In 2001, Vaziri, H. et al. have found that mammalian Sir2 negatively regulates the p53 function by physically interacting with and deacetylating the protein (2). This aspect of Sir2 function may connect various cellular damages, such as oxidative stress, to aging and carcinogenesis.
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Specificity
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Confirmed by western blotting
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Application Notes
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This antibody can be stained in formalin fixed paraffin embedded tissues with microwave pre-treatment (10mM Citrate buffer, pH6.0, 1-0 min) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 5 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting at about 3 ug/ml.
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Purification
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Antigen peptide
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml
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Storage
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Lyophilized product, 5 years at 2 8 C : Solution, 2 years at 20 C
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Restrictions
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For Research Use only
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Alternatives for antigen "Sirtuin 1 (SIRT1)", type "Antibodies"
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Hosts
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Rabbit (30), Mouse (9), Goat (2)
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Reactivities
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Human (42), Mouse (Murine) (21), Rat (Rattus) (21), Cow (Bovine) (19), Horse (Equine) (18), Rabbit (17), Chicken (16), Pig (Porcine) (4), Dog (Canine) (3), Sheep (Ovine) (1)
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Applications
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Immunofluorescence (IF) (23), Western Blotting (WB) (23), Flow Cytometry (FACS) (20), ELISA (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (8), Enzyme Immunoassay (EIA) (6), Immunoprecipitation (IP) (4), Immunohistochemistry (IHC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Chromatin Immunoprecipitation (ChiP) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Center (2), C-Term (1)
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