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Antibodies
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anti-Interferon Regulatory Factor 3 (IRF3) antibody
Interferon Regulatory Factor 3 (IRF3) antibody
| Antigen |
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| Synonyms |
IRF-3, MGC91046, C920001K05Rik, MGC94729, IRF3, irf3 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)
Western Blotting (WB) (41), Immunofluorescence (IF) (25), Flow Cytometry (FACS) (21), ELISA (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (8), Enzyme Immunoassay (EIA) (7), Immunohistochemistry (IHC) (5), ELISA (Detection) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN180209 |
| Quantity |
100 µg |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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IRF-3 (Interferon Regulatory Factor-3)
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Immunogen
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Synthetic peptide for a.a.137 a.a.150 (EDILDELLGNMVLA) of IRF-3 Purification: Affinity Purified with synthetic peptide
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Interleukin Regulatory Factor-3 (IRF-3) is the gene coding for a regulatory factor that mediates the action of IL-1, TNFalpha and LPS. It is reported that IRF-3 controls the production of interferons and chemokines in virus infected cells, by a posttranslational mechanism that involves virus induced phosphorylation, protein dimerization, cytoplasmic to nuclear translocation and stimulation of responsive genes. Furthermore, it is reported that the chemokine RANTES, an important physiological inhibitor of HIV replication, is controlled by IRF-3 activation.
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Specificity
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Confirmed by western blotting using His tag IRF-3.
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Application Notes
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This antibody can be stained in formalin fixed paraffin embedded tissues without any pre-treatment by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 1 - 5 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting at 1 5 ug/ml.
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Purification
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Antigen peptide
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml
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Storage
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Lyophilized product, 5 years at 2 8 C : Solution, 2 years at 20 C
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Restrictions
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For Research Use only
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Alternatives for antigen "Interferon Regulatory Factor 3 (IRF3)", type "Antibodies"
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Hosts
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Rabbit (39), Mouse (17)
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Reactivities
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Human (58), Mouse (Murine) (25), Rat (Rattus) (23), Cow (Bovine) (16), Horse (Equine) (16), Dog (Canine) (15), Pig (Porcine) (15), Monkey (2), Rabbit (1)
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Applications
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Western Blotting (WB) (41), Immunofluorescence (IF) (25), Flow Cytometry (FACS) (21), ELISA (12), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (8), Enzyme Immunoassay (EIA) (7), Immunohistochemistry (IHC) (5), ELISA (Detection) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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transcript variant 1 (3)
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