IRF-3 (P386 Phosphorylated) antibody

Antigen: IRF-3 (P386 Phosphorylated)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN180210

Additional Information

Immunogen: Synthetic peptide of Phosphorylated C terminal part of human IRF-3 (RVGGAS(pS)LEN) Purification: Affinity Purified with synthetic peptide

Isotype: IgG  (Matching secondary antibodies)

Description: Regulatory transcription factor IRF-3 plays a critical role in the regulation of innate immune responses, especially production of IFN-¿ /À , against viral and bacterial infections. IRF-3 is ubiquitously expressed in its inactive form in the cytoplasm. Viral infection induces phosphorylation of the Ser 386 in IRF-3 by IRF-3 kinase (IKK-i/Ã , TBK-1). This phosphorylation induces dimerization of IRF-3 and association with the coactivators CBP/p300, and the complex activates the target genes in the nucleus.

Application Details

Application Notes: This antibody can be used for western blotting in concentration of about 2 ug /mL. As this antibody is not suitable for western blotting by SDS-PAGE gel, we recommend Native-PAGE.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml

Storage: Lyophilized product, 5 years at 2 ­ 8 C : Solution, 2 years at ­20 C

Restrictions: For Research Use only

Images

anti-IRF-3 (P386 Phosphorylated) antibody

Publications

Publications: Clement, Bibeau-Poirier, Gravel et al.: "Phosphorylation of IRF-3 on Ser 339 generates a hyperactive form of IRF-3 through regulation of dimerization and CBP association." in: Journal of virology, Vol. 82, Issue 8, pp. 3984-96, 2008 (PubMed).