RET Finger Protein (RFP) antibody

Antigen: RET Finger Protein (RFP)

Synonyms: RFP, RNF76, Rfp, AW538890, TRIM27, MGC139258

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN180211

Additional Information

Immunogen: Synthetic peptide for C-terminal portion (IDGFSGHVGNHGHSMETSP) of RFP Purification: Affinity Purified with synthetic peptide

Isotype: IgG  (Matching secondary antibodies)

Description: RET finger protein (RFP) belongs to the large B-box RING finger protein family and is known to become oncogenic by fusion with RET tyrosine kinase. Although RFP is reported to be a nuclear protein that is present in the nuclear matrix, its function is largely unknown. RFP interacts with Enhancer of Polycomb (EPC) and strongly represses the gene transcription. Yeast two-hybrid assays revealed that the coiled-coil domain of RFP was associated with the EPcA domain and the carboxyl-terminal region of EPC. RFP may be involved in the epigenetic gene silencing mechanism cooperating with Polycomb group protein (ref.1).

Specificity: The antigen peptides is selected a common region between human and mouse.

Application Details

Application Notes: This antibody can be stained by Immunofluorescent techniques (ref.1). The optimal dilution is 0.1 - 1 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting at 1 ­2 ug/ml.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml.

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only