Tob (prylated) antibody

Antigen: Tob

Epitope: prylated

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180226

Additional Information

Alternative name: Tob (Phosphorylated)

Immunogen: Synthetic peptide of human Tob protein (ASSVSS(S)P(S)PPFGHS·A (S): phosphorylated) Purification: Purified with antigen peptide

Isotype: IgG  (Matching secondary antibodies)

Description: Tob is a member of an emerging family of genes with anti-proliferative function. Tob is rapidly phosphorylated at Ser 152, Ser 154, and Ser 164 by Erk1 and Erk2 upon growth-factor stimulation. Tob inhibits cell growth by suppressing cyclin D1 expression, which is canceled by Erk1- and Erk2-mediated Tob phosphorylation (1) In recent, it is reported that mutant forms of Tob, in which serines are replaced by glutamic acids to mimic phosphorylation, show a much reduced ability to inhibit the cell cycle progression to S phase from G0/G1 phase, as compared with wild-type Tob, indicating that ERK phosphorylation negatively regulates the anti-proliferative function of Tob (2).

Specificity: Confirmed by western blotting using transfectant cells

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues with microwave pre-treatment (10min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 5 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting at about 2 ug/ml.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml

Storage: Lyophilized product, 5 years at 2 ­ 8 C : Solution, 2 years at ­20 C

Restrictions: For Research Use only

Images

anti-Tob (prylated) antibody