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Antibodies
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anti-Flotillin 1 (FLOT1) (C-Term) antibody
Flotillin 1 (FLOT1) (C-Term) antibody
| Antigen |
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| Synonyms |
FLOT1, DKFZp469E0515, flot1, Rareg, flot1c, MGC84970, MGC132244, flotillin1, flot1a, flotillin-1, MGC143379, reggie2, wu:fj45g11, si:dz44o19.1 |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
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| Catalog no. |
ABIN180228 |
| Quantity |
100 µg |
| Price |
517.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Alternative name
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Flotillin-1 (C)
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Immunogen
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Synthetic peptide of common part of C terminal of rat and mouse Flotillin-1 (SQVNHNKPLRTA) Purification: Purified with antigen peptide
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Raft is rich in sphingo-gliycolipids and cholesterol, it is advocated as a membrane domain which participates in protein sorting, and it is known that many signal transduction molecules are gathering there. It is difficult to distinguish catch and identify Raft protein, unlike Cabeole which is the another micro domain of membrane, because the protein moves continuously on a cell membrane, and because the size and the structure of the protein are labile. For this reason, the marker specific to Raft was required in order to identify. Flotillin is the composition ingredient of Raft and the possibility as a marker of Raft is suggested.
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Specificity
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Reacts with Rat, Mouse and Human Flotillin-1 (Confirmed by western blotting and Immunohistochemistry)
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Application Notes
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This antibody can be stained in formalin fixed paraffin embedded tissues after microwave pretreatment (10 min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) method. The optimal dilution is about 2 ug/ml, however, the dilution rate should be optimized by each laboratories.:This antibody can be used for western blotting in concentration of about 2 ug/ml.
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Purification
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Antigen peptide
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml
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Storage
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Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20
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Restrictions
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For Research Use only
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Alternatives for antigen "Flotillin 1 (FLOT1)", type "Antibodies"
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Hosts
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Rabbit (21), Goat (4), Mouse (1)
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Reactivities
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Human (24), Mouse (Murine) (18), Rat (Rattus) (16), Cow (Bovine) (5), Dog (Canine) (5), Pig (Porcine) (4), Rabbit (3), Guinea Pig (1), Horse (Equine) (1), Sheep (Ovine) (1)
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Applications
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Flow Cytometry (FACS) (16), Immunofluorescence (IF) (15), Western Blotting (WB) (8), ELISA (2), Enzyme Immunoassay (EIA) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
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Conjugates
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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AA 365-427 (1), C-Term (1)
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