Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) antibody

Antigen: Oligodendrocyte Lineage Transcription Factor 2 (OLIG2)

Synonyms: BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19, wu:fc64g09, OLIG2, olig3.2, MGC89336

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN180229

Additional Information

Alternative name: Olig2

Immunogen: Synthetic peptide in portion of C-terminus of Human Olig2 Purification: Purified with antigen peptide

Isotype: IgG  (Matching secondary antibodies)

Description: Oligodendrocytes are defined as the cells that exist in tissue stroma of central nervous system and play a important role in maintaining function of neuron cell by making myelin wrapping around a nerve cell axon. And further, oligodendrocytes are considered as target of some disease such as autoimmunedisease and brain cancer. However, there are some problems such that the antibody to oligodendrocytes using in immunostaining has low stability and hardly react oligodendroglioma as tumor of oligodendrocytes. Olig2 is a transcription factor that controls development and differentiation of oligodendrocytes (ref. 1-3). It is reported that human Olig2 is specifically expressed in both oligodendrocytes and oligodendroglioma (ref. 4-6). This antibody recognizes with human Olig2

Specificity: Cross-reacts with Mouse and Rat

Application Details

Application Notes: This antibody can be stained in formalin fixed paraffin embedded tissues after microwave pretreatment (10 min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) method. The optimal dilution is about 0.11 ug/ml, however, the dilution rate should be optimized by each laboratories.:This antibody can be used for western blotting in concentration of about 2 ug/ml.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml

Storage: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Restrictions: For Research Use only

Images

anti-Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) antibody

Human oligodendroglioma

Mouse brain cortex