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Mesothelin (MSLN) antibody
| Antigen | Mesothelin (MSLN) |
| Synonyms | MPF, SMR, CAK1, MSLN |
| Clonality | Polyclonal |
| Host |
 Alternatives Rabbit |
| Reactivity |
Alternatives Rat (Rattus) |
| Application |
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN180247 |
| Quantity | 100 µg |
| Price | 517.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 10 to 15 Business Days |
Additional Information
| Alternative name | C-ERC/Mesothelin (306) |
| Immunogen | Synthetic peptide of the part of Rat C-ERC/Mesothelin (GKEPNVVDENLIFYQNWELEA) Purification: Purified with antigen peptide |
| Isotype | IgG (Matching secondary antibodies) |
| Description | Erc has been identified as a gene showing stronger expression in cancer-affected renal cell than in normal renal tissue in Eker rats (a rat model of renal cancer). The human homologue of the protein encoded by this gene is called MPF (megakaryocyte potentiating factor) or mesothelin. This protein is detected especially prominently in mesothelial cells, and its involvement has been suggested in the development of mesothelioma, making it a promising tumor marker. In humans, involvement of this protein has also been suggested in the development of pancreatic, ovarian and pulmonary cancers, etc. The protein is expressed as a GPI anchor-type membranous protein (about 71 kDa in molecular weight), which is thought to be digested by a furin-like protease to yield fragments about 31 kDa and 40 kDa in size. We have prepared an antibody specific to C-ERC/Mesothelin (40 kDa fragment). This antibody specifically recognizes rat C-ERC/Mesothelin which is applicable for the analysis of ERC expression in mesothelial cells, tumors, cell lines, etc. |
Application Details
| Application Notes | This antibody can be stained in formalin fixed paraffin embedded tissues by several immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 0.2 - 5 g/mL, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of 0.1 - 2 ug /mL. Reference:1. Hino O, Kobayashi E, Nishizawa M, Kubo Y, Kobayashi T, Hirayama Y, Takai S, Kikuchi Y, Tsuchiya H, Orimoto K, et al. Renal carcinogenesis in the Eker rat.J Cancer Res Clin Oncol. 1-995,121(9-10):602-5. 2. Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc Natl Acad Erc/Mesothelin.Cancer Sci. 2-007 May,98(5):659-64. |
| Purification | Antigen peptide |
| Buffer | Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 Reconstitution: 1.0 mL deionized water will be added to the product (the conc. comes up 100 ug /mL) |
| Storage | Lyophilized product, 5 years at 2 - 8 °C |
| Research Area | Cancer |
| Restrictions | For Research Use only |
Publications
| Publications |
Usami, Fukui, Kondo et al.: "Establishment and characterization of four malignant pleural mesothelioma cell lines from Japanese patients." in: Cancer science, Vol. 97, Issue 5, pp. 387-94, 2006 (PubMed).
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Alternatives
Alternatives for antigen "Mesothelin (MSLN)", type "Antibodies"
| Hosts | Rabbit (31), Mouse (12), Goat (1) |
| Reactivities | Human (34), Monkey (1), Rat (Rattus) (1) |
| Applications | Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Enzyme Immunoassay (EIA) (5), Immunoprecipitation (IP) (4), ELISA (3), Immunohistochemistry (IHC) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | C-Term (3) |
