AKT1 Substrate 1 (Proline-Rich) (AKT1S1) antibody

Antigen: AKT1 Substrate 1 (Proline-Rich) (AKT1S1)

Synonyms: Lobe, Lobel, PRAS40, AI227026, 1110012J22Rik, MGC2865, MGC81452, AKT1S1, lobe, pras40, MGC148526, fb34a04, wu:fb34a04

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Catalog no.: ABIN180254

Additional Information

Alternative name: PRAS40 (P238)

Immunogen: Synthetic peptide of the C terminal part of Human PRAS40 (DLPRPRLNTSDFQKLKRKY) Purification: Purified with antigen peptide

Isotype: IgG  (Matching secondary antibodies)

Clone: P238

Description: PRAS40 (proline-rich Akt substrate of 40 kDa) is a protein with a prolin-rich domain and appears at about 40 kDa by SDS-PAGE. Initially it was identified as a substrate of PKB/Akt, it was later shown that it is also phosphorylated by mTORC1 (a complex including scaffold protein which is necessary for mTOR and phosphorylation of substrate by mTOR). PRAS40 binds to raptor through the mediation of TOS-motif in its structure in the same way as p70 S6 kinase and 4E-P1 do as substrates of mTOR , then PRAS40 is phosphorylated by mTORC1 at Ser-183 (human), and the phosphorylated PRAS40 will be dissociated from raptor. Therefore, it is considered that PRAS40 is involved in signal-transducing pathway through the mediation of not only PKB/Akt but also mTOR.

Application Details

Application Notes: This antibody can be used for western blotting in concentration of 0.5 - 3 ug /mL. This antibody can be used for immuno-precipitation in concentration of 1 - 3 ug /mL. Reference:1. Oshiro N, Takahashi R, Yoshino K, Tanimura K, Nakashima A, Eguchi S, Miyamoto T, Hara K, Takehana K, Avruch J, Kikkawa U, Yonezawa K. The proline-rich Akt substrate of 4-0 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1. J Biol Chem. 2-007 Jul 1-3,282(28):20329-39.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 Reconstitution: 1.0 mL deionized water will be added to the product (the conc. comes up 100 ug /mL)

Storage: Lyophilized product, 5 years at 2 - 8 °C

Restrictions: For Research Use only

Images

anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) antibody