AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (pS183) antibody

Antigen: AKT1 Substrate 1 (Proline-Rich) (AKT1S1)

Synonyms: Lobe, Lobel, PRAS40, AI227026, 1110012J22Rik, MGC2865, MGC81452, AKT1S1, lobe, pras40, MGC148526, fb34a04, wu:fb34a04

Epitope: pS183

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Catalog no.: ABIN180255

Additional Information

Alternative name: PRAS40 (S183 Phosphorylated)

Immunogen: Synthetic peptide of the phosphorylated part of Human PRAS40 (QYAK(pS)LPVS) Purification: Purified with antigen peptide

Isotype: IgG  (Matching secondary antibodies)

Description: MTOR exists as two types of complexes called mTORC1 and mTORC2. mTORC1 is regulated in activity by amino acid and inhibited specifically by a immunosuppressant rapamycin. It is considered that PRAS40 is phosphorylated by mTORC1, for phosphorylation of PRAS40 (proline-rich Akt substrate of 40 kDa) which is a novel substrate of mTOR at Ser-183 (human) is induced by amino-acid stimulation and inhibited by rapamycin. It is known that mTORC1 is inhibited by AMP kinase system and then phosphorylation of PRAS40 at Ser-183 is inhibited by 2-Deoxyglucose (2DG) treatment inducing activation of AMP-kinase. It is shown that PRAS40 is dissociated from raptor which is a component of mTORC1 when it is phosphorylated by mTORC1, and it is assumed that phosphorylation of PRAS40 at Ser-183 is involved in controlling mTOR signal-transducing pathway. This antibody recognizes the phosphorylation of PRAS40 at Ser-183 (human).

Application Details

Application Notes: This antibody can be used for western blotting in concentration of 1 - 3 ug /mL. Reference:1. Oshiro N, Takahashi R, Yoshino K, Tanimura K, Nakashima A, Eguchi S, Miyamoto T, Hara K, Takehana K, Avruch J, Kikkawa U, Yonezawa K. The proline-rich Akt substrate of 4-0 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1. J Biol Chem. 2-007 Jul 1-3,282(28):20329-39.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 Reconstitution: 1.0 mL deionized water will be added to the product (the conc. comes up 100 ug /mL)

Storage: Lyophilized product, 5 years at 2 ­ 8 °C

Restrictions: For Research Use only

Images

anti-AKT1 Substrate 1 (Proline-Rich) (AKT1S1) (pS183) antibody