»
Antibodies
»
anti-Regulatory Associated Protein of MTOR, Complex 1 (RPTOR) antibody
Regulatory Associated Protein of MTOR, Complex 1 (RPTOR) antibody
| Antigen |
|
| Synonyms |
Raptor, dRaptor, raptor/CG4320, DmelCG4320, CG4320, KOG1, Mip1, KIAA1303, mKIAA1303, 4932417H02Rik, RP24-225K18.1, RGD1311784, RPTOR, raptor |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
| Catalog no. |
ABIN180256 |
| Quantity |
100 µg |
| Price |
517.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 10 to 15 Business Days |
|
Alternative name
|
Raptor (R1)
|
|
Immunogen
|
Synthetic peptide of the N terminal part of Human Raptor (MESEMLQSPLLGLGEEDEA) Purification: Purified with antigen peptide
|
|
Isotype
|
IgG (Matching secondary antibodies)
|
|
Clone
|
R1
|
|
Description
|
Raptor (regulatory associated protein of mTOR) is an about 150 kDa protein which has HEAT repeats and WD40 repeats, and it is identified as an mTOR binding protein. It is conserved over species and widely expressed in a variety of cells, and is also one of configuration factors of mTORC1/mTOR complex 1, which controls proteosynthesis and/or growth of cells along with mLST8. Raptor binds to p70 S6 kinase or 4E-BP1 (eukaryotic translation initiation factor 4E binding protein 1) which is a substrate of mTOR, through the mediation of TOS (TOR signaling) motif, and it plays an important role as a scaffold protein in phosphorylation of the substrate by mTOR.
|
|
Application Notes
|
This antibody can be used for western blotting in concentration of 1 - 3 ug /mL. This antibody can be used for immuno-precipitation in concentration of 0.25 - 3 ug /mL. Reference:1. Oshiro N, Takahashi R, Yoshino K, Tanimura K, Nakashima A, Eguchi S, Miyamoto T, Hara K, Takehana K, Avruch J, Kikkawa U, Yonezawa K. The proline-rich Akt substrate of 4-0 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1. J Biol Chem. 2-007 Jul 1-3,282(28):20329-39.
|
|
Purification
|
Antigen peptide
|
|
Buffer
|
Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 Reconstitution: 1.0 mL deionized water will be added to the product (the conc. comes up 100 ug /mL)
|
|
Storage
|
Lyophilized product, 5 years at 2 8 °C
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Regulatory Associated Protein of MTOR, Complex 1 (RPTOR)", type "Antibodies"
|
Hosts
|
Rabbit (47), Mouse (3)
|
|
Reactivities
|
Human (42), Mouse (Murine) (27), Rat (Rattus) (20), Dog (Canine) (1)
|
|
Applications
|
Immunofluorescence (IF) (27), Western Blotting (WB) (27), Flow Cytometry (FACS) (19), Enzyme Immunoassay (EIA) (7), Immunoprecipitation (IP) (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), ELISA (4), Dot Blot (Dot) (2), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
|
|
Conjugates
|
Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
N-Term (4), C-Term (3), Middle (2), pSer863 (2), Center (1), S863 (1)
|
Alternatives 
