Rictor (R1291) antibody

Antigen: Rictor (R1291)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Catalog no.: ABIN180257

Additional Information

Immunogen: Synthetic peptide of a part of Human Rictor (GSSHTLPRRAQSLKA) Purification: Purified with antigen peptide

Isotype: IgG  (Matching secondary antibodies)

Clone: R1291

Description: Rictor (rapamycin-insensitive companion of mTOR/ mAVO3) is about 200 kDa and identified as an mTOR-binding protein. Rictor is a factor that constitutes a complex with mLST8 and Sin 1 which is called mTORC2 (mTOR complex 2), and it is contrasted with raptor in mTORC1. It has a domain structure which is rather conserved over species, and these are thought to be necessary for formation of mTORC2 and binding to substrates, but then the details of their functions are not clear. Regulation of actin-cytoskeletal organization through mediation of Rho family (low molecular weight GTP-binding protein) and PKC, and phosphorylation of hydrophobic motif site at the C-terminal of PKB/Akt have been reported as effects of mTORC2 including rictor.

Application Details

Application Notes: This antibody can be used for western blotting in concentration of 1 - 3 ug /mL. This antibody can be used for immuno-precipitation in concentration of 1 - 3 ug /mL. Reference:1. Oshiro N, Takahashi R, Yoshino K, Tanimura K, Nakashima A, Eguchi S, Miyamoto T, Hara K, Takehana K, Avruch J, Kikkawa U, Yonezawa K. The proline-rich Akt substrate of 4-0 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1. J Biol Chem. 2-007 Jul 1-3,282(28):20329-39.

Purification: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 Reconstitution: 1.0 mL deionized water will be added to the product (the conc. comes up 100 ug /mL)

Storage: Lyophilized product, 5 years at 2 ­ 8 °C

Restrictions: For Research Use only

Images

anti-Rictor (R1291) antibody