Retinoblastoma 1 antibody (RB1)

Details for Product anti-RB1 Antibody No. ABIN180306, Supplier: Log in to see
Antigen
  • RB1
  • OSRC
  • RB
  • p105-Rb
  • pRb
  • pp110
  • Rb
  • Rb-1
  • zgc:154147
  • P105-RB
  • PP110
  • RB11
  • retinoblastoma 1 (including osteosarcoma)
  • retinoblastoma 1
  • Retinoblastoma-associated protein
  • RB1
  • rb
  • Rb1
  • rb1
Alternatives
anti-Human Retinoblastoma 1 antibody for BioImaging
Reactivity
Human
619
341
236
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8
5
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4
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3
2
1
1
Host
Mouse
455
165
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
28
26
25
16
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13
7
7
7
7
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7
7
5
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2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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228
167
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94
90
46
41
26
24
10
9
6
5
5
2
1
1
1
Options
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Immunogen Recombinant human retinoblastoma gene protein.
Clone 1F8
Isotype IgG1
Specificity This antibody recognises the Human retinoblastoma (Rb) gene product and specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It reacts with hyperphosphorylated and un (under) phosphorylated form of Rb protein. It shows no cross reaction with p107 and p130.
Cellular Localization: Nuclear. Does not react with Mouse and Rat.
Characteristics Synonyms: Retinoblastoma 1, Rb, p105-Rb, pRb, pp110, OSRC
Purification Purified from the ascites fluid by Protein G Chromatography
Alternative Name Retinoblastoma-Associated Protein / RB1 (RB1 Antibody Abstract)
Background Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
Molecular Weight 105 kDa
Gene ID 5925
UniProt P06400
Pathways Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
Application Notes Flow Cytometry, Immunofluorescence, Immunoprecipitation (Native and denatured): useProtein G and Ab at 2 g/mg protein lysate, Western Blotting (Ab 1-2g/mL for 2hrs at RT),Immunohistology (Formalin/paraffin: Ab 1-2g/mL for 30 min at RT) Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 1 mM EDTA, pH 8.0 for 10-20 min. followed by cooling at RT for 20 min. EDTA is better than citrate for epitopeunmasking. Recommended positive control: Ls174T, HT29, HUVEC cells. Normal colon andcarcinoma. Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, 0.09 % Sodium Azide, 0.2 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 4-8 °C for one month or at -20 °C for longer. Avoid repeated freezingand thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Retinoblastoma 1 antibody (RB1) (ABIN180306) anti-Retinoblastoma 1 (RB1) antibody
Background publications Bártek, Vojt?sek, Grand, Gallimore, Lane: "Cellular localization and T antigen binding of the retinoblastoma protein." in: Oncogene, Vol. 7, Issue 1, pp. 101-8, 1992 (PubMed).

Grand, Byrd, Grabham, Gregory, Huen, Merrick, Young, Gallimore: "The expression of the retinoblastoma gene product Rb1 in primary and adenovirus-transformed human cells." in: Oncogene, Vol. 4, Issue 11, pp. 1291-8, 1989 (PubMed).