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Cyclin-Dependent Kinase 1 (CDK1) antibody
| Antigen | Cyclin-Dependent Kinase 1 (CDK1) |
| Synonyms |
CDK1, CDC28A, MGC111195, DKFZp686L20222, Cdc2, Cdc2a, p34 |
| Clonality | Monoclonal (A17) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Amphibian, Bird (Avian), Mammalian, Xenopus laevis |
| Application |
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|
| Catalog no. | ABIN180309 |
| Quantity | 0.1 mg (0.2 mg/ml) |
| Price | 550.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Characteristics | Synonyms: p34 protein kinase, Cell division control protein 2 homolog, Cyclin-dependent kinase 1 |
| Alternative name | CDK1 / CDC2 |
| Swiss-Prot | P06493 |
| Immunogen | C-Terminal 2/3rds of Xenopus cdc2 expressed in E. coli |
| Isotype | IgG2a (Matching secondary antibodies) |
| Clone | A17 |
| Description | P34cdc2(a catalytic subunit of Maturation Promoting Factor) plays a crucial role during celldivision and is most active during mitosis. It is a serine/threonine kinase, which isactivated by cyclin, by dephosphorylation of tyrosine residues. p34cdc2 is inactivated by atyrosine kinase. |
| Specificity | This antibody supports the kinase activity. It inhibits the activation of p34cdc2 kinase bycyclins. Cellular Localization: cytoplasmic. Mol. Wt of Antigen: 34kDSpecies: Human, Mouse, Rat, Guinea Pig, Woodchuck, Xenopus, and ChickenOthers not tested. |
Application Details
| Application Notes | Immunofluorescence. Immunoprecipitation (Native and denatured): use Protein A and Ab at2 g/mg protein lysate. Kinase Assay. Western Blotting (1-2g/ml for 2hrs at RT). Immunohistology (Formalin/paraffin): 2-4 ug/ml for 30min at RT. Staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0. Recommendedpositive control: HeLa, MAD109, PC12 or NIH-3T3 Cells, Tonsil, or Lymph Node. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. |
| Concentration | 0.2 mg/ml |
| Buffer | 10mM PBS buffer with 0.09% sodium azide as preservative and 0.2% BSAas stabilizer |
| Storage | Store the antibody at 4-8°C for one month or at -20°C for longer. Avoid repeated freezingand thawing. Shelf life: one year from despatch. |
| Research Area | Cell Cycle |
| Restrictions | For Research Use only |
Images
| Formalin-fixed, paraffin-embedded human tonsil stained with cdk1 Ab (Cat.AM00203) using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of lymphocytes in the germinal center. |
Alternatives
Alternatives for antigen "Cyclin-Dependent Kinase 1 (CDK1)", type "Antibodies"
| Hosts | Rabbit (94), Mouse (15) |
| Reactivities | Human (103), Rat (Rattus) (54), Mouse (Murine) (53), Xenopus laevis (3), Chicken (2), Cow (Bovine) (2), Sheep (Ovine) (2), Fruit Fly (Drosophila melanogaster) (1), Rabbit (1) |
| Applications | Western Blotting (WB) (95), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (36), Enzyme Immunoassay (EIA) (27), Immunohistochemistry (IHC) (19), ELISA (16), Immunofluorescence (IF) (13), Immunoprecipitation (IP) (13), Dot Blot (Dot) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Flow Cytometry (FACS) (2), ELISA (Detection) (1) |
| Epitopes | pTyr15 (15), pThr161 (12), C-Term (6), pThr14 (3), N-Term (2), pSer39 (2), Ab-14 (1), Center K200 (1), S39 (1), T14 (1), pT14 (1), pT161 (1) |
