Transglutaminase 2, Tissue (TGM2) antibody

Antigen: Transglutaminase 2, Tissue (TGM2)

Synonyms: TG2, TGC, GNAH, G-ALPHA-h, TgaseII, TGM2, fc12b04, MGC136700, wu:fc12b04, zgc:136700, zgc:101081

Clonality: Monoclonal (CUB 7402)

Host: Mouse

Reactivity: Dog (Canine), Guinea Pig, Human, Rabbit

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN180314

Additional Information

Characteristics: Synonyms: TGase C, TGase-H, Tissue transglutaminase, Protein-glutamine gamma-glutamyltransferase2

Alternative name: Transglutaminase-2 (TGM2)

Swiss-Prot: P21980

Immunogen: Purified guinea pig liver TGase II

Isotype: IgG1  (Matching secondary antibodies)

Clone: CUB 7402

Description: Transglutaminase II catalyzes calcium-dependent post-translational modification ofproteins by formation of an isopeptide bond within or between polypeptide chains. It isalso known as TGC, tTG, type II-, Gh, cytosolic-, liver-, endothelial-, erythrocyte-,cellular-transglutaminase. Different tissues and cell types express varying amounts oftissue transglutaminase with a markedly hugh expression in rheumatoid lesions. It isimplicated in programmed cell death, signal transduction, drug-resistance, cell growth,endocytosis, insulin secretion, cell adhesion, cataract formation, and wound healing.

Specificity: Cellular Localization: cytoplasm, cell membrane, and extracellular matrix. Mol. Wt. of Antigen: 77-85kD. Epitope: aa 447-478. Species: Human, Dog, Rabbit, and Guinea Pig. Does not react with Mouse and Rat. Others not tested.

Application Details

Application Notes: Immunofluorescence. Immunoprecipitation (Native and denatured): use Protein G and Ab at 2 µg/mg proteinlysate. Western Blotting: Ab 1-2 µg/ml for 2hrs at RT. Immunohistology (Formalin/paraffin): Ab 1-2 µg/ml for 30 min at RT, staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0. Recommended positive control: HUVEC Cells, Breast Carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Buffer: 10 mM PBS with 0.09% Sodium Azide as preservative and 0.2% BSA asstabilizer

Storage: Store at 2-8°C. Shelf life: one year from despatch.

Research Area: Enzymes, Extracellular Matrix

Restrictions: For Research Use only

Images

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Transglutaminase II Ab (Cat. AM00208) using peroxidase-conjugate and DAB chromogen. Note cell membrane and cytoplasmic staining of tumor and endothelial cells.