CNPase (CNP) antibody
| Antigen |
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| Synonyms |
CNP1, Cnp1, Cnp-1, CNPase, CNPF, CNPI, CNPII, CNP, sb:cb662, wu:fd44a05, wu:fi35d08, si:ch73-158e11.4, DKFZp469F1421 |
| Clonality |
Monoclonal (11-5B) |
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Host
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Reactivity
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 Alternatives Cow (Bovine), Dog (Canine), Cat (Feline), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
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Application
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Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN180337 |
| Quantity |
0.1 mg (0.2 mg/ml) |
| Price |
530.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: CNP, CNP1, Oligodendrocyte Marker, 2', 3'-cyclic-nucleotide 3'-phosphodiesterase
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Alternative name
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CNPase
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Gene ID
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1267
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Swiss-Prot
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P09543
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Immunogen
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Purified human 2-3-cyclic nucleotide 3-phosphodiesterase (CNPase)
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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11-5B
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Description
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CNPase (2,3-Cyclic Nucleotide 3-Phosphodiesterase) exists in two forms: 46kDa (CNP1) and48kDa (CNP2). It is expressed at high levels by oligodendrocytes in the central nervoussystem and by Schwann cells in the peripheral nervous system and is virtually absent inother cell types. CNPase activity is decreased in demyelinating diseases such as multiplesclerosis. CNPase gene is localized to chromosome 17q21, very near the BRCA1 familialbreast cancer gene.
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Specificity
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This antibody is useful for early specific identification of oligodendrocytes and Schwanncells. Cellular Localization: Cell Membrane. Mol. Wt. of Antigen: 46kD (CNP1) and 48kD(CNP2). Species: Human, Monkey, Cow, Sheep, Goat, Pig, Cat, Dog, Rabbit, Rat, and Mouse. Doesnot react with Guinea Pig and Chicken. Others not tested.
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Application Notes
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Immunofluorescence. Western Blotting. Immunohistochemistry on frozen and paraffin embedded sections: Ab 2-4 ug/ml for 30min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mMcitrate buffer, pH 6.0. Recommended positive control: brain. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.2 mg/ml
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Buffer
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10 mM PBS buffer with 0.09% sodium azide as preservative and 0.2% BSAas stabilizer
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Storage
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Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freezethaw cycles! Shelf life: one year from despatch.
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Research Area
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Neurology, Glia marker
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Restrictions
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For Research Use only
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Alternatives for antigen "CNPase (CNP)", type "Antibodies"
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Hosts
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Rabbit (13), Mouse (3), Chicken (1)
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Reactivities
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Human (16), Mouse (Murine) (8), Rat (Rattus) (8), Rabbit (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (15), ELISA (Detection) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), ELISA (2), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), ELISA (Capture) (1), Immunofluorescence (IF) (1), Immunohistochemistry (IHC) (1), Immunoprecipitation (IP) (1)
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Epitopes
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AA 68-84 (1)
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