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Cyclin-Dependent Kinase 6 (CDK6) antibody

Details for Product No. ABIN180342
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Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(187), (86), (62), (38), (38), (24), (12), (1)
Host
Mouse
(130), (60)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(123), (64), (54), (38), (37), (35), (30), (24), (18), (6), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN180342
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Immunogen Purified recombinant cdk6 protein.
Clone DCS-90
Isotype IgG1
Characteristics Synonyms: PLSTIRE, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE
Purification Protein G affinity purified immunoglobulin fraction
Alternative Name CDK6
Background P40 or cyclin-dependent kinase 6 (p40cdk6), also known as PLSTIRE, is part of a family ofproteins designated as cdks which are critical regulators of cell cycle progression. Theprototype member of this family, p34cdc2, and a related protein cdk2, function late in thecycle while cdk4 and cdk6 are critically involved in G1 to S progression.
UniProt Q00534
Research Area Cell Cycle
Application Notes Immunoprecipitation (Native and denatured): use Protein G and Ab at 2g/mg proteinlysate. Not suitable for Kinase assay. Western Blotting: Ab 1-2g/ml for 2hrs at RT. Immunocytochemistry and Immunohistology on frozen sections. Recommended positivecontrol: Ls174T Cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10mM PBS with 0.09% sodium azide as preservative and 0.2% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 2-8°C for up to one month or in aliquots at -20°C for longer. Avoid repeated freezethaw cycles! Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Lukas, Jensen, Bartkova et al.: "Immunohistochemical analysis of the D-type cyclin-dependent kinases Cdk4 and Cdk6, using a series of monoclonal antibodies." in: Hybridoma, Vol. 18, Issue 3, pp. 225-34, 1999 (PubMed).

Validation Images
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