Paxillin (PXN) antibody

Details for Product No. ABIN180343
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Human, Rat (Rattus)
(383), (166), (156), (19), (2), (1), (1), (1)
(369), (44), (2)
Clonality (Clone)
Monoclonal ()
(9), (9), (9), (7), (7), (7), (7), (7), (7), (7), (7), (2), (2), (2), (1), (1)
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(324), (106), (97), (70), (65), (54), (26), (22), (14), (7), (4), (4), (3), (3), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN180343
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Immunogen Purified recombinant full-length human paxillin protein.
Clone 5H11
Isotype IgG1
Specificity Highly specific for Paxilin.
Cellular Localization: cell membrane, cytoplasmic.
Characteristics Synonyms: FLJ16691
Purification Affinity Chromatography on Protein G.
Alternative Name Paxillin / PXN
Background Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk).Synonyms: FLJ16691
Gene ID 5829
UniProt P49023
Application Notes Immunofluorescence. Immunoprecipitation (Native): use Protein G and Ab at 2 g/mgprotein lysate. Western Blotting (1-2 μg/mL for 2hrs at RT). Immunohistology(Formalin/paraffin, 1-2 μg/mL for 30 min at RT, Staining of formalin-fixed tissues REQUIRESboiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 mins followed by cooling at RT for 20 mins, Recommended positive control: HeLa Cells, Colon Carcinoma).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 10 mM PBS with 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freeze thaw cycles!
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Paxillin (PXN) antibody anti-Paxillin (PXN) antibody
Background publications Hanks, Polte: "Signaling through focal adhesion kinase." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 19, Issue 2, pp. 137-45, 1997 (PubMed).

Leventhal, Shelden, Kim et al.: "Tyrosine phosphorylation of paxillin and focal adhesion kinase during insulin-like growth factor-I-stimulated lamellipodial advance." in: The Journal of biological chemistry, Vol. 272, Issue 8, pp. 5214-8, 1997 (PubMed).

de Curtis, Malanchini: "Integrin-mediated tyrosine phosphorylation and redistribution of paxillin during neuronal adhesion." in: Experimental cell research, Vol. 230, Issue 2, pp. 233-43, 1997 (PubMed).

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