Dystrophin (DMD) antibody

Antigen: Dystrophin (DMD)

Synonyms: DMD, MGC155049, Dmd, MGC83347, DKFZp459C1629, dmd, MGC79631, DKFZp468A1620, Dp71, cb664, zfDYS, im:6911785, zgc:110165, BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, mdx, pke, Dp427, DXSmh7, DXSmh9, RATDMD, DNADMD1, CG17750, CG31175, CG7240, CG7243, CG7344, DLP, DLP1, DLP186, DLP2, DLP3, DmDLP, DmDYS, Dp205, GI3046716, dmDp186, DmelCG34157, CG34157

Clonality: Monoclonal (1808)

Host: Mouse

Reactivity: Amphibian, Chicken, Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN180361

Additional Information

Alternative name: Dystrophin / DMD

Swiss-Prot: P11532

Immunogen: Acetylcholine Receptor (AChR)-enriched membranes and peripheral membrane proteinsfrom Torpedo nobiliana electric organ. Remarks: Mol. Wt of Antigen: 427 kDa

Isotype: IgG1  (Matching secondary antibodies)

Clone: 1808

Description: Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helixrod-containing proteins. It is absent or greatly reduced in individuals with the X-linkedrecessive Duchenne's muscular dystrophy disorder, as well as in mice with the mdx(murine muscular dystrophy) mutation.

Specificity: This antibody is highly specific to Dystrophin and shows no cross-reaction with C-Protein(an isoform of alpha-Actinin), alpha-Actin, or Human muscle Spectrin. Cellular Localization: cell membrane. Species: Human, Mouse, Rat, Chicken, Xenopus, and Torpedo. Others not tested.

Synonyms: DMD, MGC155049, Dmd, MGC83347, DKFZp459C1629, dmd, MGC79631, DKFZp468A1620, Dp71, cb664, zfDYS, im:6911785, zgc:110165, BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, mdx, pke, Dp427, DXSmh7, DXSmh9, RATDMD, DNADMD1, CG17750, CG31175, CG7240, CG7243, CG7344, DLP, DLP1, DLP186, DLP2, DLP3, DmDLP, DmDYS, Dp205, GI3046716, dmDp186, DmelCG34157, CG34157

Application Details

Application Notes: Electron Microscopy and Immunofluorescence (see Ref.1). Immunohistology (Formalin/Paraffin): Use Ab 2-4 µg/ml for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0. Recommended Positive Control: Skeletal Muscle. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Buffer: 10 mM PBS, pH 7.4, with 0.09% Sodium Azide as preservative and 0.2%BSA as stabilizer.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze thaw cycles! Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Formalin-fixed, paraffin-embedded human skeletal muscle stained with Dystrophin Ab (Cat. AM00254) using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of muscle fibers.