L1 Cell Adhesion Molecule (L1CAM) antibody

Antigen: L1 Cell Adhesion Molecule (L1CAM)

Synonyms: S10, HSAS, MASA, MIC5, SPG1, CAML1, CD171, HSAS1, N-CAML1, l1cam-a, MGC80658

Clonality: Monoclonal (UJ127-11)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN180399

Additional Information

Characteristics: Synonyms: N-CAM L1, CAML1, MIC5, Neural cell adhesion molecule L1

Alternative name: CD171 / L1CAM

Swiss-Prot: P32004

Immunogen: Homogenous suspension of 16 week Human foetal brain. Remarks: Mol. Wt. of Antigen: 220-240 kDa

Isotype: IgG1  (Matching secondary antibodies)

Clone: UJ127-11

Description: The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS(hydrocephalus-stenosis of the aqueduct of Sylvius), 2) MASA (mental retardation,aphasia, shuffling gait, adductus thumbs), and 3) SPG1 (spastic paraplegia). The L1, neuralcell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation,neural migration and in mediating neuronal differentiation. Expression of L1 protein isrestricted to tissues arising from neuroectoderm.

Specificity: This antibody is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin. It does not bind to pediatric or adultbrain. Cellular localization: Cell membrane. Species: Human. Others not tested.

Application Details

Application Notes: Immunoprecipitation (Native and Denatured): Use at 2 µg/mg protein lysate (Use ProteinG). Western Blotting: Use at 1-2 µg/ml for 2 hrs at RT. Immunohistology (Formalin/Paraffin): Use at 1/100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Recommended Positive Control: IMR-5 Cells, Neuroblastoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Buffer: 10 mM PBS, pH 7.4, with 0.2% BSA as stabilizer and 0.09% Sodium Azideas preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Figure 1. Formalin-fixed, paraffin-embedded human schwannoma stained with L1 Cell Adhesion Molecule Ab (ABIN180399) using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.