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Details for Product No. ABIN180429

Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) antibody

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Antigen
Synonyms ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5, CASP-8, Mch5, caspase-8, zgc:92075, casp8-A, xCaspase-8, casp8, CASP8
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 2 references available
Catalog no. ABIN180429
Quantity 0.1 mg
Price
643.50 $   Plus shipping costs $45.00
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Immunogen Recombinant full length human Caspase 8 protein. Remarks: Mol. Wt. of Antigen: 55 kDa
Clone 8CSP03
Isotype IgG1
Characteristics Synonyms: CASP-8, CASP8, MCH5, CAP4, ICE-like apoptotic protease 5, MORT1-associated CED-3homolog, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, Apoptotic cysteineprotease, Apoptotic protease Mch-5
Purification Protein G chromatography
Alternative Name Caspase-8
Background Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DEDdomain present in tandem in the proform of the caspase 8 protein. Recruitment of caspase8 to the Fas receptor results in oligomerization of the caspase 8 protein, which in turndrives its autoactivation through “self-cleavage”. Activated caspase 8 then activates otherdownstream caspases including caspase 9, thereby commiting the cell to undergoapoptosis.
UniProt Q14790
Research Area Apoptosis/Necrosis
Application Notes Immunoprecipitation(2g/mgprotein lysate, Denatured verified, Use Protein G). Western Blotting (2-4g/mlfor 2hrs at RT). Positive Control: Jurkat cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Muzio, Chinnaiyan, Kischkel et al.: "FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death--inducing signaling complex." in: Cell, Vol. 85, Issue 6, pp. 817-27, 1996 (PubMed).

Hu, Vincenz, Ni et al.: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997 (PubMed).

Alternatives for antigen "Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8)", type "Antibodies"
Hosts (159), (60), (6), (1)
Reactivities (198), (68), (57), (30), (13), (12), (12), (12), (5), (2), (1), (1), (1), (1)
Applications (190), (75), (48), (40), (37), (22), (14), (13), (10), (7), (6), (4), (2), (2), (2), (2), (2), (1), (1)
Conjugates (11), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (29), (13), (6), (5), (5), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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