GPR125 antibody (AA 1098-1109)

Catalog No. ABIN1804467

Product Details anti-GPR125 Antibody

Target: GPR125 (G Protein-Coupled Receptor 125 (GPR125))

Binding Specificity: AA 1098-1109

Reactivity: Human, Mouse, Cow, Bat, Hamster, Horse, Monkey, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This GPR125 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human GPR125.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CTNKSASSFKNS from an internal region of human GPR125 (NP_660333.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Bovine, Bat, Horse, Rabbit (100%), Panda, Dog, Pig, Opossum, Turkey, Platypus, Xenopus (92%), Chicken (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000), WB (0.5 - 1.5 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 140kD band observed in Human Ovary lysates (calculated MW of 146kD according to NP_660333.2). Recommended concentration: 0.5-1.5 μg/mL. An additional band of unknown identity was also consistently observed at 33kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GPR125

Target: GPR125 (G Protein-Coupled Receptor 125 (GPR125))

Alternative Name: ADGRA3 / GPR125 (GPR125 Products)

Background: Name/Gene ID: ADGRA3
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRA3, GPR125, TEM5L, G protein-coupled receptor 125, PGR21

Gene ID: 166647

NCBI Accession: NP_660333

UniProt: Q8IWK6