Tppp antibody (AA 21-35)

Catalog No. ABIN1804484

Product Details anti-Tppp Antibody

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Binding Specificity: AA 21-35

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Tppp antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TPPP.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DPSKDRAAKRLSLES from an internal region of human TPPP (NP_008961.1). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Dog (93%), Panda, Pig (87%), Mouse, Rat, Hamster, Bat (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:32000), WB (0 - 0.01 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 26kD band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kD according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10,313(20):4091-106, PMID: 18028908). Recommended concentration: 0.001-0.01 μg/mL. An additional band of unknown identity was also consistently observed at 17kD. This band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Tppp

Target: Tppp (Tubulin Polymerization Promoting Protein (Tppp))

Alternative Name: TPPP (Tppp Products)

Synonyms: TPPP/p25 antibody, TPPP1 antibody, p24 antibody, p25 antibody, p25alpha antibody, 2900041A09Rik antibody, AI849835 antibody, RGD1310121 antibody, tubulin polymerization promoting protein antibody, TPPP antibody, Tppp antibody

Background: Name/Gene ID: TPPP

Synonyms: TPPP, 25 kDa brain-specific protein, p25-alpha, p25, p25alpha, p24, TPPP1

Gene ID: 11076

NCBI Accession: NP_008961

UniProt: O94811