Pituitary Tumor-Transforming 1 (PTTG1) antibody

Details for Product No. ABIN180460
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Antigen
Reactivity
Human
(80), (31), (25), (12), (1)
Host
Mouse
(57), (20), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(49), (35), (22), (22), (20), (10), (10), (3), (3), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN180460
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Immunogen Human recombinant full length Pds1 protein. Remarks: Mol. Wt. of Antigen: 42 kDa
Clone DCS-280
Isotype IgG2a
Characteristics Synonyms: PTTG, EAP1, TUTR1, Pituitary tumor-transforming gene 1 protein, Esp1-associated protein,Tumor-transforming protein 1
Purification Protein A Chromatography.
Alternative Name Securin / PTTG1
Background Pds1 is an anaphase inhibitor and plays important role in DNA damage and spindle checkpoint pathways. Pds1 inhibits sister chromatid separation by binding and inhibiting Esp1,cystein protease that causes cleavage of the cohesin Scc1 that binds the sister chromatidstogether. Pds1 is responsible for targeting of Esp1 to nucleus and its binding to the spindle. Degradation of Pds1 occurs shortly before anaphase, which liberates Esp1 and is aprerequisite for anaphase entry. Pds1 is targeted for degradation by ubiquitinationmediated by cyclosome/anaphase-promoting complex (APC) functioning as a ubiquitinligase. In response to DNA damage Chk1 phosphorylates Pds1 to stabilize it against theAPC mediated destruction, hence preventing the entry of such a cell into anaphase.
Gene ID 9232
UniProt O95997
Application Notes Western Blotting (Not verified). Immunohistology (formalin/paraffin): Ab 1-2 ug/ml for 30 min at RT. Staining offormalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for10-20 min followed by cooling at RT for 20 min. Recommended positive control: HeLa Cells, Tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10mM PBS, pH 7.4 containing 0.2% BSA as stabilizer and 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Pituitary Tumor-Transforming 1 (PTTG1) antibody anti-Pituitary Tumor-Transforming 1 (PTTG1) antibody
Background publications Jacq, Alt-Mörbe, Andre et al.: "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." in: Nature, Vol. 387, Issue 6632 Suppl, pp. 75-8, 1997 (PubMed).

Wang, Liu, Wang et al.: "Pds1 phosphorylation in response to DNA damage is essential for its DNA damage checkpoint function." in: Genes & development, Vol. 15, Issue 11, pp. 1361-72, 2001 (PubMed).

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