MUC16 antibody (Extracellular Domain)

Catalog No. ABIN1804607

Product Details anti-MUC16 Antibody

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC16 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes the ovarian cancer mucin CA125 (also known as MUC16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90 % of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces. CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Recognizes the epitope specificity group B, similar to the M-11 antibody. May be used to detect CA125 in ELISA assays.

Purification: Ion exchange chromatography

Immunogen: CA125 antigen purified from human ovarian carcinoma.

Clone: X325

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB

Usage: ELISA: This product may be used in an indirect ELISA or as a detection antibody in a sandwich ELISA together with LS-B2829 as a capture reagent.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris buffered saline, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: MUC16 / CA125 (CA125 Products)

Synonyms: CA125 antibody, 1110008I14Rik antibody, Ca125 antibody, Gm1480 antibody, RGD1308422 antibody, MUC16 antibody, mucin 16, cell surface associated antibody, mucin 16 antibody, mucin-16 antibody, MUC16 antibody, Muc16 antibody, LOC476718 antibody, LOC712735 antibody

Background: Name/Gene ID: MUC16

Synonyms: MUC16, CA125 ovarian cancer antigen, CA125, CA-125, MUC-16, Mucin-16

Gene ID: 94025

UniProt: Q8WXI7