CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (AA 1-80)

Details for Product anti-CDKN1A Antibody No. ABIN180466, Supplier: Log in to see
Antigen
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • cyclin-dependent kinase inhibitor 1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a
  • cki1
  • CDKN1A
  • LOC100348489
  • Cdkn1a
Alternatives
anti-Rat (Rattus) CDKN1A antibody for Cytometry by Time of Flight
Epitope
AA 1-80, N-Term
50
39
27
20
18
18
18
18
15
15
15
15
14
13
12
12
9
8
7
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
535
186
126
30
23
10
6
5
4
2
1
1
1
1
1
1
1
1
Host
Mouse
310
267
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CDKN1A antibody is un-conjugated
30
29
29
22
15
15
13
13
9
9
7
7
7
7
7
7
7
7
7
6
6
6
6
4
4
4
4
4
4
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
330
212
172
167
136
101
67
62
53
33
30
13
7
6
6
2
1
1
Options
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Immunogen Full length human recombinant p21 protein. Remarks: Mol. Wt. of antigen: 21 kDaEpitope: aa 1-80
Clone CP74
Isotype IgG2b
Specificity This antibody detects p21/WAF1.
Cellular Localization: Nuclear.
Characteristics Synonyms: CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1,CDK-interacting protein 1, Melanoma differentiation-associated protein 6
Purification Protein A Chromatography.
Alternative Name CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)
Background P21WAF1/Cip1/Sdi1/Pic1 is a tumor suppressor protein. Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. It also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
Gene ID 1026
UniProt P38936
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
Application Notes Flow Cytometry. Immunoprecipitation (Native verified): Use Protein A, 2 μg/mg protein lysate. Western blotting: 1-2 μg/mL for 2hrs at RT. Immunohistology (Formalin/Paraffin): Use at 1/50 for 30 min. Staining of formalin-fixedtissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for for 10-20 minfollowed by cooling at RT foir 20 min. Positive Control. Raji, PC12 cells, Colon carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4 containing 0.2 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (AA 1-80) (ABIN180466) anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-80), (N-Term) antibody
Background publications Chen, Cipriano, Sarkar, Ware, Arenkiel: "P53-independent induction of p21(waf1) pathway is preserved during tumor progression." in: International journal of oncology, Vol. 7, Issue 4, pp. 889-93, 2011 (PubMed).

Buckley, Sweeney, Hamilton, Sini, Manning, Nicholson, deFazio, Watts, Musgrove, Sutherland: "Expression and amplification of cyclin genes in human breast cancer." in: Oncogene, Vol. 8, Issue 8, pp. 2127-33, 1993 (PubMed).