THBS3 antibody (AA 936-950)

Catalog No. ABIN1804710

Product Details anti-THBS3 Antibody

Target: THBS3 (Thrombospondin 3 (THBS3))

Binding Specificity: AA 936-950

Reactivity: Mouse, Human, Pig, Cow, Dog, Horse, Monkey, Bat, Rat, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This THBS3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human THBS3.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide CNDTVPEDFEPFRRQ from the C-terminus of human THBS3 (NP_009043.1). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig (100%), Xenopus, Zebrafish (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 50  kDa band in Human Lung and in lysates of cell line A549 after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 104  kDa according to NP_009043.1. The 50  kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P with formalin fixed tissues and HIER antigen retrieval. The antibody does not work in IHC using samples fixed in 4 % PFA.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for THBS3

Target: THBS3 (Thrombospondin 3 (THBS3))

Alternative Name: THBS3 / Thrombospondin 3 (THBS3 Products)

Synonyms: thbs3 antibody, tsp3 antibody, zgc:103461 antibody, tsp-3 antibody, THBS3 antibody, TSP3 antibody, Thbs-3 antibody, thrombospondin 3a antibody, thrombospondin 3 antibody, thrombospondin 3 L homeolog antibody, thbs3a antibody, THBS3 antibody, thbs3.L antibody, thbs3 antibody, Thbs3 antibody

Background: Name/Gene ID: THBS3

Synonyms: THBS3, Thrombospondin-3, Thrombospondin 3, TSP3

Gene ID: 7059

NCBI Accession: NP_009043

UniProt: P49746