ZIC3 antibody (AA 178-189)

Catalog No. ABIN1804747

Product Details anti-ZIC3 Antibody

Target: ZIC3 (Zic Family Member 3 (ZIC3))

Binding Specificity: AA 178-189

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Monkey, Guinea Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ZIC3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human ZIC3. This antibody is expected to NOT cross-react with other ZIC proteins.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HVDNNQVHLGLR from an internal region of human ZIC3 (NP_003404.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Bovine, Dog, Elephant, Panda, Horse, Rabbit, Porcine, Opossum, Guinea pig (100%), Bat, Platypus (92%), Zebra finch (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Preliminary experiments gave an approx 70kD band in Human, Muse and Rat Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.6kD according to NP_003404.1. The 70kD band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZIC3

Target: ZIC3 (Zic Family Member 3 (ZIC3))

Alternative Name: ZIC3 (ZIC3 Products)

Synonyms: HTX antibody, HTX1 antibody, VACTERLX antibody, ZNF203 antibody, RGD1561261 antibody, zic3-A antibody, Bn antibody, Zic family member 3 antibody, Zic family member 3 S homeolog antibody, zinc finger protein of the cerebellum 3 antibody, ZIC3 antibody, Zic3 antibody, zic3.S antibody

Background: Name/Gene ID: ZIC3

Synonyms: ZIC3, Heterotaxy 1, HTX1, Zic family member 3, Zinc finger protein ZIC 3, ZNF203, HTX, VACTERLX, Zinc finger protein 203

Gene ID: 7547

NCBI Accession: NP_003404

UniProt: O60481