Laminin, gamma 1 (LAMC1) antibody

Details for Product No. ABIN180492
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Antigen
Reactivity
Guinea Pig, Human, Rat (Rattus)
(50), (22), (20), (1), (1)
Host
Mouse
(38), (24), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (1), (1)
Application
Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(53), (38), (19), (13), (7), (7), (7), (5), (3), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN180492
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Immunogen Rat glomerular basement membrane. Remarks: Mol. Wt. of Antigen: ~210 kDa
Clone D18
Isotype IgG2a
Specificity This antibody detects Laminin B2/gamma-1.
Cellular Localization: Basement membrane.
Characteristics Synonyms: LAMC1, LAMB2, Laminin subunit gamma-1, Laminin B2 chain
Purification Protein A Chromatography.
Alternative Name Laminin gamma 1
Background Laminin, a major structural glycoprotein of the basement membranes, is an attachment and survival factor for epithelial cells and can also promote the growth of epithelial cells. Laminin plays an important role in both the structural organization of basement membranes and in the anchorage of cells. Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha (LAMA1), beta (LAMB1), and gamma (LAMC1), formerly A, B1, and B2, respectively. Several isoforms of each chain have been identified. Laminin gamma 1 is the most ubiquitously expressed laminin subunit. Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. They are ubiquitously present in basement membrane (BM) along with entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms.Synonyms: LAMB2, LAMC1, Laminin B2 chain, Laminin subunit gamma-1
Gene ID 3915
UniProt P11047
Research Area Neurology
Application Notes Immunohistology (Formalin/Paraffin): Use at 2-4 μg/mL for 30 min at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1 mg/mL PBS for5 minutes at 37 °C. Immunoprecipitation and Western Blotting not verified. Positive Control: Ls174T cells, Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
anti-Laminin, gamma 1 (LAMC1) antibody anti-Laminin, gamma 1 (LAMC1) antibody
Background publications Green, Hunter, Chan et al.: "Synthesis and assembly of the synaptic cleft protein S-laminin by cultured cells." in: The Journal of biological chemistry, Vol. 267, Issue 3, pp. 2014-22, 1992 (PubMed).

Sanes, Engvall, Butkowski et al.: "Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere." in: The Journal of cell biology, Vol. 111, Issue 4, pp. 1685-99, 1990 (PubMed).

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