Laminin, gamma 1 (LAMC1) antibody

Antigen: Laminin, gamma 1 (LAMC1)

Synonyms: LAMB2, MGC87297, B2e, sly, LAMC1, lamb2

Clonality: Monoclonal (D18)

Host: Mouse

Reactivity: Guinea Pig, Human, Rat (Rattus)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN180492

Additional Information

Characteristics: Synonyms: LAMC1, LAMB2, Laminin subunit gamma-1, Laminin B2 chain

Alternative name: Laminin gamma 1

Swiss-Prot: P11047

Immunogen: Rat glomerular basement membrane. Remarks: Mol. Wt. of Antigen: ~210 kDa

Isotype: IgG2a  (Matching secondary antibodies)

Clone: D18

Description: Laminin, a major structural glycoprotein of the basement membranes, is an attachmentand survival factor for epithelial cells and can also promote the growth of epithelial cells. Laminin plays an important role in both the structural organization of basementmembranes and in the anchorage of cells. Laminin is a heterotrimeric extracellular matrixprotein consisting of 3 chains: alpha (LAMA1), beta (LAMB1), and gamma (LAMC1), formerlyA, B1, and B2, respectively. Several isoforms of each chain have been identified. Laminingamma 1 is the most ubiquitously expressed laminin subunit. Laminins are large heterotrimeric, noncollagenous glycoproteins composed of alpha, beta,and gamma chains. They are ubiquitously present in basement membrane (BM) along withentactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan(HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described inmany types of human and animal epithelial neoplasms.

Specificity: This antibody detects Laminin B2/gamma-1. Cellular Localization: Basement membrane. Species: Human, Rat and Guinea pig.

Application Details

Application Notes: Immunohistology (Formalin/Paraffin): Use at 2-4 µg/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with Protease XXV at 1 mg/ml PBS for5 minutes at 37°C. Immunoprecipitation and Western Blotting not verified. Positive Control: Ls174T cells, Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml

Buffer: 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% Sodium Azide aspreservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Neurology

Restrictions: For Research Use only

Images

Formalin-fixed, paraffin-embedded human skin stained with ABIN180492 using peroxidase-conjugate and AEC chromogen. Note basement membrane staining of skin.