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Stratifin antibody (SFN)

Details for Product anti-SFN Antibody No. ABIN180589, Supplier: Log in to see
Antigen
  • Er
  • Mme1
  • Ywhas
  • YWHAS
Alternatives
anti-Human Stratifin antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Human
181
53
38
10
8
8
5
4
4
3
3
2
2
2
2
1
1
1
Host
Mouse
139
35
17
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Stratifin antibody is un-conjugated
9
9
7
6
6
6
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
160
79
63
47
31
18
18
16
11
5
3
2
1
1
Supplier
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Immunogen Recombinant human 14-3-3 sigma proteinRemarks: Mol. Wt. of Antigen: 26-30 kDa
Clone 1433S01
Isotype IgG1
Specificity This antibody is highly specific to 14.3.3 sigma and shows no cross-reaction with other isoforms of 14.3.3.
Cellular Localization: Cytoplasmic.
Characteristics Synonyms: Stratifin, HME1, Epithelial cell marker protein 1
Purification Protein G Chromatography.
Alternative Name 14-3-3 Protein sigma / SFN (SFN Antibody Abstract)
Background 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis, including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 sigma shares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130, p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and in vivo.Synonyms: Epithelial cell marker protein 1, HME1, Stratifin
Gene ID 2810
UniProt P31947
Pathways p53 Signaling
Application Notes Immunofluorescence. Western Blotting: 1 μg/mL for 2 hrs at RT. Immunoprecipitation: use Protein G, 2 g/mg protein lysate. Immunohistology (Formalin/paraffin): 2-4 μg/mL for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: HT29 cells. Skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4 containing 0.2 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Stratifin antibody (SFN) (ABIN180589) anti-Stratifin (SFN) antibody
Background publications Villaret, Wang, Dillon, Xu, Sivam, Cheever, Reed: "Identification of genes overexpressed in head and neck squamous cell carcinoma using a combination of complementary DNA subtraction and microarray analysis." in: The Laryngoscope, Vol. 110, Issue 3 Pt 1, pp. 374-81, 2000

Hermeking, Lengauer, Polyak, He, Zhang, Thiagalingam, Kinzler, Vogelstein: "14-3-3 sigma is a p53-regulated inhibitor of G2/M progression." in: Molecular cell, Vol. 1, Issue 1, pp. 3-11, 1998