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Stratifin (SFN) antibody

Details for Product No. ABIN180589
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Antigen
Reactivity
Human
(119), (37), (30), (17), (14), (6), (5), (3), (2), (1), (1), (1), (1)
Host
Mouse
(88), (29), (6), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(112), (46), (41), (37), (17), (16), (16), (10), (9), (4), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN180589
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Immunogen Recombinant human 14-3-3 sigma proteinRemarks: Mol. Wt. of Antigen: 26-30 kDa
Clone 1433S01
Isotype IgG1
Characteristics Synonyms: Stratifin, HME1, Epithelial cell marker protein 1
Purification Protein G Chromatography.
Alternative Name 14-3-3 protein sigma / SFN
Background 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolicproteins. 14-3-3 proteins bind to and influence the activities of a diverse group ofmolecules involved in signal transduction, cell cycle regulation and apoptosis, includingRaf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are stronglyinfluenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 sigmashares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130,p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and invivo.
UniProt P31947
Application Notes Immunofluorescence. Western Blotting: 1 ug/ml for 2 hrs at RT. Immunoprecipitation: use Protein G, 2 g/mg protein lysate. Immunohistology (Formalin/paraffin): 2-4 ug/ml for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: HT29 cells. Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4 containing 0.2% BSA as stabilizer and 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch
Expiry Date 12 months
Supplier Images
anti-Stratifin (SFN) antibody anti-Stratifin (SFN) antibody
Background publications Hermeking, Lengauer, Polyak et al.: "14-3-3 sigma is a p53-regulated inhibitor of G2/M progression." in: Molecular cell, Vol. 1, Issue 1, pp. 3-11, 1998 (PubMed).

Villaret, Wang, Dillon et al.: "Identification of genes overexpressed in head and neck squamous cell carcinoma using a combination of complementary DNA subtraction and microarray analysis." in: The Laryngoscope, Vol. 110, Issue 3 Pt 1, pp. 374-81, 2000 (PubMed).

Validation Images
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