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Details for Product No. ABIN180589

Stratifin (SFN) antibody

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Antigen
Synonyms YWHAS, Er, Mme1, Ywhas
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 2 references available
Catalog no. ABIN180589
Quantity 0.1 mg
Price
643.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Recombinant human 14-3-3 sigma proteinRemarks: Mol. Wt. of Antigen: 26-30 kDa
Clone 1433S01
Isotype IgG1
Characteristics Synonyms: Stratifin, HME1, Epithelial cell marker protein 1
Purification Protein G Chromatography.
Alternative Name 14-3-3 protein sigma / SFN
Background 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolicproteins. 14-3-3 proteins bind to and influence the activities of a diverse group ofmolecules involved in signal transduction, cell cycle regulation and apoptosis, includingRaf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are stronglyinfluenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 sigmashares cyclin-Cdk2 binding motifs with different cell cycle regulators, including p107, p130,p21Cip1, p27Kip1, and p57Kip2 and is associated with cyclin-Cdk complexes in vitro and invivo.
UniProt P31947
Application Notes Immunofluorescence. Western Blotting: 1 ug/ml for 2 hrs at RT. Immunoprecipitation: use Protein G, 2 g/mg protein lysate. Immunohistology (Formalin/paraffin): 2-4 ug/ml for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: HT29 cells. Skin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4 containing 0.2% BSA as stabilizer and 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch
Expiry Date 12 months
Background publications Hermeking, Lengauer, Polyak et al.: "14-3-3 sigma is a p53-regulated inhibitor of G2/M progression." in: Molecular cell, Vol. 1, Issue 1, pp. 3-11, 1998 (PubMed).

Villaret, Wang, Dillon et al.: "Identification of genes overexpressed in head and neck squamous cell carcinoma using a combination of complementary DNA subtraction and microarray analysis." in: The Laryngoscope, Vol. 110, Issue 3 Pt 1, pp. 374-81, 2000 (PubMed).

Alternatives for antigen "Stratifin (SFN)", type "Antibodies"
Hosts (68), (24), (7), (2)
Reactivities (95), (33), (29), (17), (14), (6), (5), (3), (2), (1), (1), (1), (1)
Applications (88), (37), (35), (27), (13), (13), (10), (8), (3), (2), (1), (1)
Conjugates (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (14), (9), (8), (8), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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